Earticle

초록

영어

Sildenafil citrate, a potent and selective inhibitor of phosphodiesterase type-5, is used clinically to treat erectile dysfunction and pulmonary ar-terial hypertension. We investigated the effect of sildenafil citrate on brain central fatigue through serotonin (5-hydroxytryptamine, 5-HT) synthesis after exhaustive swimming exercise in rats. The rats in the sildenafil citrate-treated groups received sildenafil citrate orally once a day for 14 consecutive days at respective dosage. On the 14 days after starting experiment, each animal was submitted to swimming test with intensity equivalent to overload. The exhaustion was defined as a state in which coordinated movements did not return to the water surface for breathing within 10 sec. Immunohistochemistry for 5-HT, tryptophan hy-droxylase (TPH), and western blot for serotonergic type 1A (5-HT1A) re-ceptor and 5-HT transporter (5-HTT) were performed. Exhaustive swim-ming exercise increased 5-HT and TPH expressions in the dorsal raphe and sildenafil citrate suppressed 5-HT and TPH expressions in the ex-haustive swimming exercise rats. Exhaustive swimming exercise in-creased 5-HT1A receptor and 5-HTT expressions in the dorsal raphe and sildenafil citrate suppressed 5-HT1A receptor and 5-HTT expres-sions in the exhaustive swimming exercise rats. The significant sup-pressing effect appeared in the 20-mg/kg sildenafil citrate. Sildenafil ci-trate might be proposed as a potential ergogenic aid through anticentral fatigue.

목차

Abstract
INTRODUCTION
MATERIALS AND METHODS
Animals
Exhaustive swimming exercise
Tissue preparation
Immunohistochemistry for 5-HT and TPH
Western blot analysis
Statistical analysis
RESULTS
Effect of sildenafil citrate on exhaustive swimming time
Effect of sildenafil citrate on 5-HT expression in the dorsal raphe
Effect of sildenafil citrate on TPH expression in the dorsal raphe
Effect of treadmill exercise on 5-HT1A receptor and 5-HTT expression in the dorsal raphe
DISCUSSION
CONFLICT OF INTEREST
ACKNOWLEDGMENTS
REFERENCES

키워드

저자

• Sang-Seo Park [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Tae-Woon Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Chang-Ju Kim [ Department of Physiology, College of Medicine, Kyung Hee University, Seoul, Korea ]
• Seo-Youn Hong [ Department of Sports Medicine, Soonchunhyang University, Asan, Korea ]
• Bo-Kyun Kim [ Department of Emergency Technology, College of Health Science, Gachon University, Incheon, Korea ]
• Young-Je Sim [ Department of Physical Education, Kunsan National University, Gunsan, Korea ]
• Mal-Soon Shin [ College of Culture and Sports, Division of Global Sport Studies, Korea University, Sejong, Korea ] Corresponding Author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  JER [Journal of Exercise Rehabilitation]

• 간기

  격월간

• pISSN

  2288-176X

• eISSN

  2288-1778

• 수록기간

  2013~2026

• 등재여부

  SCOPUS,KCI 등재

• 십진분류

  KDC 517 DDC 613