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Comparisons of Developmental Potential and Gene Expression Level in Porcine Nuclear Transfer, Parthenogenetic and Fertilized Embryos

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 30 No 2 (2006.06)바로가기
  • 페이지
    pp.125-133
  • 저자
    Jung-Gon Kim, B. Mohana Kumar, Sung-Keun Cho, Sun-A Ock, Byeong-Gyun Jeon, S. Balasubramanian, Gyu-Jin Rho, Sang-Yong Choe
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A36259

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원문정보

초록

영어
This study was conducted to detect the apoptosis incidence in blastocysts and to compare the abundance of Bax, Bcl2L1, VEGF and FGFR2 in in vitro fertilized (IVF), parthenogenetic (PAT) and nuclear transfer (NT) embryos. Oocytes matured for 40 hr were enucleated and reconstructed with confluenced fetal fibroblasts (FFs) derived from a ～45 day fetus. Reconstructed eggs were then fused with 2 DC pulses (2.0 kV/cm, 30 μsec) and cultured with 7.5 μg/ml cytochalasin B for 3 hr. Parthenotes (PAT) were produced with the same electric strength and culture for NT eggs. The embryos were cultured in NCSU-23 medium at 39℃, 5% CO2, 5% O2 in air. In 3 runs, set of 10 embryos at the 4-cell to blastocyst stages were used to extract total RNA for analyzing the gene expression patterns of pro-apoptotic (Bax), anti-apoptotic (Bcl2L1), vasculogenesis (VEGF), implantation (FGFR2III) using real-time quantitative PCR. Cleavage and blastocyst rates were significantly higher (P<0.05) in IVF and PAT (79.3±8.5 and 25.5±6.1, and 85.0±6.4 and 38.6±5.5, respectively) than NT counterparts (65.1±5.2 and 15.6±3.0, respectively). Significantly higher (P<0.05) total cells were observed in IVF controls and PAT (34.7±5.8 and 38.1±4.1) than NT embryos (24.8±3.2). Apoptosis index was significantly lower (P<0.05) in IVF than NT embryos. The Relative abundances (RA) of Bax and VEGF were significantly higher (P<0.05) at blastocyst stage in NT than IVF control. The RA of Bcl2L1 and FGFR2III were significantly higher (P<0.05) at blastocyst stage in IVF than NT. The present study observed the abnormal gene expressions in NT embryos at various developmental stages, suggesting certain clues to find out the cause of the low efficiency of NT to term.

목차

INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

키워드

Porcine Apoptosis Nuclear transfer Gene expression

저자

  • Jung-Gon Kim [ Institute of Animal Medicine, College of Veterinary Medicine ]
  • B. Mohana Kumar [ Institute of Animal Medicine, College of Veterinary Medicine ]
  • Sung-Keun Cho [ Division of Applied Life Science, Gyeongsang National University ]
  • Sun-A Ock [ Division of Applied Life Science, Gyeongsang National University ]
  • Byeong-Gyun Jeon [ Division of Applied Life Science, Gyeongsang National University ]
  • S. Balasubramanian [ Department of Clinics, Madras Veterinary College, TamilNadu Veterinary and Animal Sciences University ]
  • Gyu-Jin Rho [ Institute of Animal Medicine, College of Veterinary Medicine ]
  • Sang-Yong Choe [ Institute of Animal Medicine, College of Veterinary Medicine ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

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