Earticle

현재 위치 Home

Morphological Assessment of Ovulated and In Vitro Immature Canine Oocytes and Biological Availability according to the Size at Different Reproductive Stages

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & developmental biology 바로가기
  • 통권
    Volume 30 No 2 (2006.06)바로가기
  • 페이지
    pp.99-105
  • 저자
    Mohammad-Shamim Hossein, Min-Kyu Kim, Goo Jang, Yuda-Heru Fibrianto, Huyn-Joo Oh, Hye-Jin Kim, Joung-Joo Kim, Sung-Keun Kang, Byeong-Chun Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A36255

※ 기관로그인 시 무료 이용이 가능합니다.

4,000원

원문정보

초록

영어
The growing oocytes become progressively capable of resuming meiosis, and full meiotic competence appear when they are about 80% of the size of fully grown oocytes. As hormonal influences vary at different stages of reproductive cycle, the size of oocytes may vary according to the reproductive stages. The present study was designed to compare the diameter between the ovulated and freshly collected immature canine oocytes. The ovulated oocytes were collected 72 hr after ovulation by oviductal tube flushing by laparotomy under general anesthesia. Immature oocytes were collected by ovarian slicing method. Diameter of all oocytes was measured directly using epiflurescence microscope with a calibrated micro-eyepiece micrometer at ×200 magnification. The thickness of zona pellucida and diameter of cytoplasm were measured separately and recorded. A total of 2209 zona intact oocytes were collected, among them 628 from anestrus, 675 from follicular, 838 from luteal and 68 by fallopian tubes flushing methods. The average number of oocytes was 104.7, 168.8, 119.7 and 11.3 for anestrus, follicular, luteal and fallopian tubes flushing methods, respectively. The average diameters of the ooplasm and oocyte were significantly varied in different reproductive stages as well as with ovulated oocytes (P<0.05). The average diameter of ooplasm and oocyte was 115.6 and 127.7, 143.0 and 162.0, 134.6 and 150.6, 159.6 and 185.6 for anestrus, follicular, luteal and ovulated oocytes, respectively. Highest number of oocytes with larger diameter could be collected from the follicular and luteal stages. In conclusion, the follicular and luteal ovaries are the best sources of oocytes for canine IVM.

목차

INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
ACKNOWLEDGEMENT
REFERENCES

키워드

Reproductive stage Maturation Oocyte Diameter Canine

저자

  • Mohammad-Shamim Hossein [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Min-Kyu Kim [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Goo Jang [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Yuda-Heru Fibrianto [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Huyn-Joo Oh [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Hye-Jin Kim [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Joung-Joo Kim [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Sung-Keun Kang [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]
  • Byeong-Chun Lee [ Department of Theriogenology, College of Veterinary Medicine, Seoul National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & developmental biology
  • 간기
    계간
  • pISSN
    1738-2432
  • eISSN
    2288-0151
  • 수록기간
    1977~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & developmental biology Volume 30 No 2

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.