Earticle

초록

영어

The possibility of producing transgenic embryos expressing the green fluorescence protein (GFP) gene have been evaluated after transfer of exogenous gene into the porcine zygote cytoplasm using the intracytoplasm sperm injection (ICSI) as gene delivery method. For DNA binding to sperm heads, 0.05% Triton X-100 or Lipofectin was used. After injection of the sperm bound to DNA by means of Lipofectin or Triton X-100 triturate, the blastocyst formation rates on day 6 were not significantly different from that of ICSI only group (18.8, 19.2 and 25.3%). In terms of GFP expression, more embryos were in GFP form in Triton X-100 group than in Lipofectin group (40.6 vs 36.4%), while percentage of non-mosaic embryos expressing the GFP gene in all blastomere was higher (P<0.05) in Lipofectin group than in Triton X-100 group (4.2 vs 0.9%). ICSI embryos derived from sperm treated with Lipofectin/DNA complex was transferred into 3 recipients and were collected by uterine flushing on days 5, 7 and 15 after embryo transfer, and then GFP expression was observed by a fluorescence microscopy. Over 26% of the collected embryos were normally expressed GFP gene. These results suggest that foreign gene transfer method with DNA bound sperm caused minimal damage to structure of oocytes that can result to full development of porcine embryos. This was confirmed in this study when the embryos that were transferred after ISCI of DNA bound sperm had a normal development and gene expression until preimplantation.

목차

INTRODUCTION
MATERIALS AND METHODS
RESULTS
DISCUSSION
REFERENCES

키워드

저자

• Joo-Hee Han [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]
• Sungwoo Kim [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]
• Poongyeon Lee [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]
• Chun-Gyu Park [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]
• Hyun-Gi Lee [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]
• Boh-Suk Yang [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]
• Ki-Hyeong Rhee [ College of Industrial Science, Kongju National University ]
• Chang-Hyung Lee [ Department of animal Science, Korea National Agricultural College, RDA ]
• Hoon-Taek Lee [ Animal Resources Research Center, Konkuk University ]
• Won-Kyong Chang [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]
• Jin-Ki Park [ Animal Biotechnology Division, National Livestock Research Institute, RDA ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & developmental biology

• 간기

  계간

• pISSN

  1738-2432

• eISSN

  2288-0151

• 수록기간

  1977~2018

• 십진분류

  KDC 527 DDC 636