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Genetic Diversity Evaluation of Thamnocalamus spathiflorus (Trin.) Munro Accessions through Morphological and Randomly Amplified Polymorphic DNA (RAPD) Markers

첫 페이지 보기
  • 발행기관
    강원대학교 산림과학연구소 바로가기
  • 간행물
    Journal of Forest and Environmental Science KCI 등재 바로가기
  • 통권
    제35권 제2호 (2019.06)바로가기
  • 페이지
    pp.90-101
  • 저자
    Chandrakant Tiwari, Meena Bakshi, Dinesh Gupta
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A357193

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원문정보

초록

영어
Biodiversity refers to the total number and variation among species of flora and fauna of an area. Due to tremendous biotic especially anthropogenic pressure these natural resources are being vanishing. In present study genetic diversity among accessions of Thamnocalamus spathiflorus was evaluated. A total of 51 vegetative characters and 42 primers (10-mer) were screened. Out of 42 screened primers, 28 polymorphic primers were selected for further analysis. A total of 263 bands were recorded as polymorphic whereas 48 bands were monomorphic. The resolving power (Rp) of 28 Randomly Amplified Polymorphic DNA (RAPD) primers ranged from 4.6 (OPE08) to 17.6 (OPA11). The polymorphic information content (PIC) value ranged from 0.21 (OPAH09) to 0.44 (OPG02). The result revealed high degree of genetic relatedness (56 to 80%). Cluster analysis revealed two major clusters both for morphology as well as RAPD. Unlike morphological characterization, the accession (D5) from Bahli, Rampur, Shimla (H.P.) was clustered separately from the others in RAPD cluster analysis. Accessions with closed locality grouped together through RAPD marker system however analogy was recorded for morphological traits. The study conducted reflects the utility of RAPD technique for species identification and phylogenetic studies in bamboo for conducting bamboo breeding program.

목차

Abstract
Introduction
Materials and Methods
Experimental site
Experimental material
Morphological study
Genomic DNA isolation and RAPD study
Statistical analysis
Results
Morphological variability
Cluster analysis
RAPD analysis
Cluster analysis
Discussion
Conclusion
Acknowledgements
References

키워드

polymorphism randomly amplified polymorphic DNA (RAPD) polymorphic information content (PIC) value Thamnocalamus spathiflorus cluster analysis

저자

  • Chandrakant Tiwari [ Department of Forest Biology & Tree Improvement, College of Forestry, Banda University of Agriculture & Technology (BUAT), Banda 210001, India ] Corresponding Author
  • Meena Bakshi [ Plant Physiology Discipline, Botany Division, Forest Research Institute, Dehradun 248006, India ]
  • Dinesh Gupta [ Department of Silviculture and Agroforestry, College of Forestry, Banda University of Agriculture & Technology (BUAT), Banda 210001, India ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    강원대학교 산림과학연구소 [Institute of Forest Science Kangwon National University]
  • 설립연도
    1975
  • 분야
    농수해양>임학
  • 소개
    강원대학교부설산림과학연구소(이하 “연구소”라 한다)는 산림에 관한 제반 학술적 연구를 통하여 산림자원의 효용을 밝히고 임업 및 임산업의 발전에 기여함을 목적으로 한다.

간행물

  • 간행물명
    Journal of Forest and Environmental Science [산림과학연구]
  • 간기
    계간
  • pISSN
    2288-9744
  • eISSN
    2288-9752
  • 수록기간
    1981~2026
  • 등재여부
    KCI 등재
  • 십진분류
    KDC 526 DDC 634

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