Earticle

초록

영어

Alcohol-induced hangover and liver diseases are considered as a cause of serious health morbidity, mortality and socio-economic burden worldwide. In recent years, many researchers explored abundant numbers of natural products related with hepatoprotective effect against oxidative damage. In this study we investigated the protective effects of urushiol-free enzyme-treated lacquer tree (Rhus verniciflua sap) extract (ELTE) on alcohol-induced in Sprague Dawley (SD) rat model. Rats were randomly divided into 3 groups; negative control without treatment (NeC), positive control group (PC) treated with anti-hangover drink (Condition), and experimental group orally treated with urushiol-free ELTE. Experimental materials were administered to each SD rat accordingly 25 mg/kg for 5 d via gavage. At 5th day after treatment of experimental materials, each group received a dose of ethanol (4 g/kg body weight) and blood serum sample was assessed after 1 h and 5 h to investigate immune-redox and hangover markers. Our results showed blood lymphocytes levels were significantly lower in ELTE group than NeC group. Likewise, ROS generation was significantly reduced in ELTE group as compared to all other groups. In line, alcohol concentration in blood was significantly reduced in PC and ELTE groups as compared with NeC group. Collectively, our results suggest that urushiol-free ELTE might be an excellent potential candidate against acute alcohol-induced hangover relief and liver protection.

목차

ABSTRACT
Introduction
Material and Methods
1. Animals
2. Preparation of enzyme-treated lacquer tree extract (ELTE)
3. Experimental design
4. White blood cell (WBC) and its differential count
5. Determination of total ROS activity
6. Measurement of antioxidant enzyme activities
7. Measurement of alcohol concentration
8. Measurement of acetate concentration
9. Measurement of liver enzyme markers
10. Measurement of cytokines levels
11. Statistical analysis
Results
1. Effects of ELTE in the alcohol-treated SD rats on the body weight
2. Effects of ELTE in WBC differential count
3. Effects of ELTE on the total ROS level
4. Effects of ELTE on the antioxidant
5. Effects of ELTE on the alcohol concentration in serum
6. Effects of ELTE on the acetate level in serum
7. Effects of ELTE on serum levels of liver enzymes (ALT and AST)
8. Effects of ELTE on inflammatory cytokine levels in serum
Discussion
Conclusion
Acknowledgements
Conflict of Interest
Human and animal rights
References

키워드

저자

• Johny Bajgai [ Department of Environmental Medical Biology, Department of Global Medical Science ]
• Eun-Sook Jeong [ Department of Environmental Medical Biology, Department of Global Medical Science ]
• Ailyn Fadriquela [ Department of Environmental Medical Biology, Department of Global Medical Science ]
• Dong-Heui Kim [ Department of Environmental Medical Biology ]
• Cheol-Su Kim [ Department of Environmental Medical Biology ]
• Soo-Ki Kim [ Department of Microbiology, Yonsei University Wonju College of Medicine, Wonju, Gangwon 26426, Republic of Korea ]
• Kyu-Jae Lee [ Department of Environmental Medical Biology, Institute for Poverty Alleviation and International Development, Yonsei University, Wonju Campus, Wonju, Gangwon 26493, Republic of Korea ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  한국물학회지 [Korean Journal of Waters]

• 간기

  부정기

• pISSN

  2233-4653

• 수록기간

  2010~2021

• 십진분류

  KDC 539 DDC 628