Earticle

초록

영어

Plant tissue culture techniques offer quick methods of regeneration of plants of medicinal importance but the survival chances of such plants are always questionable when shifted to the in vivo conditions. The present study enumerates the micromorphological developments in the leaves of in vitro regenerated and field transferred plantlets of Oldenlandia umbellata. The leaves developed in vitro after 4th subcultures of multiplication phase and after 6 weeks of field transferred plants were used. Statistically significant differences in the number of stomata, veins, raphides, crystals and trichome density per square mm were observed. The improvements in stomatal apparatus and density (decreased from 41.85 to 32.20), developments in leaf architectural parameters and emergence of defense mechanism through increased numbers of raphides (8 to 15), crystals and trichomes (13.5 to 18.2) proved acclimation of tissue culture raised plantlets from in vitro to the in vivo environments lead to 100 % success in field establishment of the plantlets. The in vitro induced foliar abnormalities (changes in stomata, venation pattern, vein density, trichomes, crystals etc.) were repaired while hardening of plantlets in the greenhouse and finally in the field. The observed micromorphological response of leaves under altered environmental conditions could help in determination of proper stage of field transfer and prediction of survival percentage of in vitro regenerated O. umbellata plantlets.

목차

Abstract
Introduction
Materials and Methods
Study area and collection of plants
In vitro propagation of O. umbellata
Foliar micromorphological studies
Experimental design, data collection and analysis
Results and Discussion
Establishment of cultures of O. umbellata
Foliar micromorphological studies
Conclusion
Conflict of Interest
Acknowledgments
Author Contribution
References

키워드

저자

• Revathi Jayabal [ Department of Botany, Kanchi Mamunivar Center for Postgraduate Studies, Puducherry 605008, India ]
• Latha Rasangam [ Department of Botany, Kanchi Mamunivar Center for Postgraduate Studies, Puducherry 605008, India ]
• Manokari Mani [ Department of Botany, Kanchi Mamunivar Center for Postgraduate Studies, Puducherry 605008, India ]
• Mahipal Singh Shekhawat [ Department of Botany, Kanchi Mamunivar Center for Postgraduate Studies, Puducherry 605008, India ] Corresponding author

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Journal of Forest and Environmental Science [산림과학연구]

• 간기

  계간

• pISSN

  2288-9744

• eISSN

  2288-9752

• 수록기간

  1981~2026

• 등재여부

  KCI 등재

• 십진분류

  KDC 526 DDC 634