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Poster Presentation : Transgenesis / Xenotransplatation

Efficient System to Establish Pig Cell Lines to Prevent Immunodeficiency using CRISPR/Cas9 System

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.135-135
  • 저자
    Dong-Hwan Kim, Jin Seop Ahn, Wu-Sheng Sun, Seong-Hong Jang, Ga-Ram Kim, Jeong-Woong Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347610

원문정보

초록

영어
For the pig to human organ transplantation, immune rejection is the biggest problem. It is known that T cell is associated with immune rejection and it is necessary to produce severe combined immune deficiency (SCID) pigs to reduce the rejection. Previous studies show that IL2RG and RAG2 mainly function on T cell development which is mainly functions on immune system. Especially, IL2RG is for the T cell and NK cell development, and RAG2 is for maturation of T and B cells. In this study, to produce IL2RG and RAG2 deficient cell lines, we designed specific sgRNAs which target pig IL2RG and RAG2, respectively, using CRISPR/Cas9 system. Each of the GFP tagged targeting vectors were constructed and transfected into pig fibroblasts, respectively or both, to establish single or double knock-out cell lines. After transfection, GFP positive cells were isolated and many single cells were freely seeded and cultured individually. To analysis genomic types of the cells, we amplified and analyzed the targeting regions on DNA. As a result, 3 knock-out cell lines were established for IL2RG and RAG2, respectively. Also, for the double knock-out cell lines, 1 cell line was analyzed by sequencing. By CRISPR/Cas9 system, we established diverse IL2RG and/or RAG2 knock-out cell lines and the cells are planning on producing SCID pigs.

키워드

CRISPR/Cas9 SCID Immune rejection IL2RG RAG2

저자

  • Dong-Hwan Kim [ Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ]
  • Jin Seop Ahn [ Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ]
  • Wu-Sheng Sun [ Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ]
  • Seong-Hong Jang [ Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ]
  • Ga-Ram Kim [ Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ]
  • Jeong-Woong Lee [ Biotherapeutics Translational Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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