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Poster Presentation : Sperm / Male Reproduction

The SM, Domestically Developed Semen Extender for Stallions, is as Effective as INRA 96, as a Base Solution for Stallion Semen Freezing Extender

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.112-113
  • 저자
    Jongsin Won, Heejun Jung, Jung-Ho Son, Minjung Yoon
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347594

원문정보

초록

영어
The freezing semen extender of stallions has been used routinely for artificial insemination (AI) of the mares. The INRA96 was often used as a base solution for freezing extender for stallion semen in addition of egg yolk and glycerin. In this study, the possible use of SM, domestically developed semen extender for fresh and cool semen, as a base solution for freezing semen extender was tested. The semen was collected from 3 Thoroughbred stallions (an average age of 6.66±0.19) using CSU artificial vagina. Semen was diluted in SM or INRA96 at 1:2 ratio and centrifuged to remove the supernatant in where seminal plasma was placed. Sperms were resuspended with freezing medium prepared with 2% egg yolk and 2.5% glycerol added to SM or INRA96 and frozen in 0.5 cc straws. For thawing process, straws were immersed in the 37℃ water bath for 30 sec. After thawing, total and progressive motility, membrane integrity, viability and mitochondria membrane potential were measured to compare the freezing efficiency of these base solutions. Statistical analysis was performed using SAS program. Total and progressive motility, membrane integrity, membrane integrity, viability, and mitochondria membrane potential of frozen/thawed sperms with SM based freezing medium were not significantly different with those of sperms frozen/thawed with INRA 96 based freezing medium. This result suggests that SM is as effeiceint as INRA96 as a base solution for freezing medium. However, the membrane integrity (28±6.6), viability (22.8±3.8), and mitochondria membrane potential (24.6±11.8) of frozen/thawed sperms with SM were very low. Also total (32.3±4.2) and progressive motility (1.72± 0.52) of these sperms were not acceptable for commercial use. In conclusion, the efficiency of SM as a base solution for freezing medium of stallion semen is comparable with INR96.

키워드

Semem freezing extender Sperm Stallions Frozen/thawed semen

저자

  • Jongsin Won [ Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Korea ]
  • Heejun Jung [ Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Korea ]
  • Jung-Ho Son [ Noah Biotech Inc., Cheonan, Korea ]
  • Minjung Yoon [ Department of Animal Science and Biotechnology, Kyungpook National University, Sangju, Korea, Department of Horse, Companion and Wild Animal Science, Kyungpook National University, Sangju, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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