Earticle

초록

영어

With the global demand for dairy protein for consumption growing annually, there has been increasing activity in the research field of dairy protein synthesis and production. From a manipulation perspective, it is more difficult to use live cattle for laboratory studies on the production of milk as well as of dairy protein such as casein, as compared with using laboratory animals like rodents. Therefore, we aimed to develop a mouse model of bovine mammary alveolar ducts for laboratory-scale studies. We studied the formation of the bovine mammary gland ductal structure by transplanting the MAC-T bovine alveolar cell line into mice. MAC-T cells (1×107) were suspended in Matrigel and injected into the dorsal tissue of 8-week-old male BALB/C nude mice. Histological analysis of tissue dissected from the MAC-T cell-transplanted mice after 6 weeks showed the typical morphology of the tubuloalveolar female gland, as well as glands made up of branching ducts that were surrounded by smooth muscle with small alveoli budding off the ducts. In addition, the epithelial markers CK14 and CK18 were expressed within the duct-like structure. Prolactin was detected in the duct interior in these CK14+ and CK18+ cells but not in the non-transplanted MAC-T cells. These results showed that duct-like tissue had been successfully formed after 6 weeks of transplantation of the CK14+ and CK18+ MAC-T cells into mice dorsal tissue. This mouse model will be a useful tool for further research on the bovine mammary gland.

키워드

저자

• Woo Tae Ha1 [ Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea ]
• Hyun Jung Park [ Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea ]
• Won-Young Lee [ Department of Food Bioscience, College of Biomedical & Health Science, Konkuk University, Chung-ju, Republic of Korea ]
• Ha Yeon Jeong [ Department of Animal Resources Development Dairy Science Division, National Institute of Animal Science, RDA, Cheonan, Korea ]
• Hyuk Song [ 3Department of Animal Resources Development Dairy Science Division, National Institute of Animal Science, RDA, Cheonan, Korea ]

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간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636