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Poster Presentation : Reproductive Endocrinology

Establishment of Co-culture System to Enhance Milk Protein Expression in Mac-T Cells with Preadipocyte 3T3-L1 Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.102-102
  • 저자
    Won-Young Lee, Hyun Jung Park, Ha Yeon Jeong, Hyuk Song
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347587

원문정보

초록

영어
Mammary gland development is critically dependent on the interaction between the stromal and the epithelial compartments within the gland. In this study, we established a co-culture of bovine mammary epithelial Mac-T cells and murine preadipocyte 3T3-L1 cells. Mac-T cells were co-cultured with 3T3-L1 cells for four days and production of milk proteins was induced for three days. After seven days of co-culturing, the number of alveolar-like colonies in the presence of 3T3-L1 cells was significantly higher than that in control group. Expression levels of αS1-casein and β-casein mRNAs were significantly increased by co-culturing with 3T3-L1 cells. In addition, casein protein production was significantly higher in the co-culture of Mac-T cells with 3T3-L1 cells than in control Mac-T cells. Substances that induced casein production in Mac-T cells also stimulated adipogenesis in 3T3-L1 cells. We suggest that a co-culture system of bovine mammary epithelial cells and preadipocyte cells is an efficient method for in vitro milk protein production.

키워드

Mammary gland Alveolar Casein Adipocyte

저자

  • Won-Young Lee [ Department of Food Bioscience, College of Biomedical & Health Science, Konkuk University, Chung-ju, Republic of Korea ]
  • Hyun Jung Park [ Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea ]
  • Ha Yeon Jeong [ Department of Animal Resources Development Dairy Science Division, National Institute of Animal Science, RDA, Cheonan, Korea ]
  • Hyuk Song [ Department of Stem Cell and Regenerative Biology, Konkuk University, Seoul, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & Developmental Biology(Supplement) Volume 41 No 2 Supplement

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