Earticle

초록

영어

Fatty acid synthase (FASN) is an enzyme that catalyzes the synthesis of long chain fatty acid. During development, FASN plays a role in growth rather than the energy storage pathways. In this study, we hypothesis that knockout of FASN may affect the early embryonic development through induction of the endoplasmic reticulum (ER) stress. The function of FASN was studied using the Crispr/Cas9 technology. We found that FASN knockout induced ER stress by generation of reactive oxygen species (ROS), further resulted in the activation of adaptive unfolded protein response (UPR), caused the splicing of XBP1. In addition, FASN knockout increased ATF4 and CHOP expression, influenced phosphorylation of PERK. At last, Ca2+ was released from the ER and then taken up by the mitochondrial and influenced mitochondrial function, initiated apoptosis. These results demonstrated that FASN knockout induced generation of ROS, which mediated the activation of UPR via the ER and subsequent apoptosis in porcine early embryos.

키워드

저자

• Jing Guo [ Department of Animal Science, Chungbuk National University, Cheongju ]
• Ying-Jie Niu [ Department of Animal Science, Chungbuk National University, Cheongju ]
• Kyung-Tae Shin [ Department of Animal Science, Chungbuk National University, Cheongju ]
• Nam-Hyung Kim [ Department of Animal Science, Chungbuk National University, Cheongju ]
• Xiang-Shun Cui [ Department of Animal Science, Chungbuk National University, Cheongju ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636