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Poster Presentation : Oocyte Maturation / Embryonic Development

Zinc is crucial for the Asymmetric Cell Division by Control of Localization and Activity of Spire in Mouse Oocyte Maturation

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.95-95
  • 저자
    Yu-Jin Jo, In-won Lee, Seong-min Jeong, Jeong-woo Kwon, Nam-Hyung Kim, Suk Namgoong
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347582

원문정보

초록

영어
Zinc (Zn2+) is one of essential factors during mammalian oocyte maturation and fertilization. Previous studies showed that depletion of cellular Zn by metalion chelator impair asymmetric division of oocyte. But the detailed mechanism of these phenomena is unclear. We found that depletions of zinc by cell-permeable heavy metal chelator N,N,N',N'- tetrakis(2-pyridylmethyl)ethane-1,2-diamine (TPEN) caused the decrease of cytoplasmic actin mesh level. Spire2-GFP is co-localized with zinc at the cortex and intracellular vesicle. By the treatment of TPEN, number of Spire2-GFP decorated vesicle is drastically decreased, indicating that Zn2+ is essential for the localization of the spire in mouse oocyte. Two putative zinc-binding regions were located in the C-terminal part of Spire2. Mutations of zinc binding site on spire abolish its localization at the intracellular vesicle. Over expression of C-terminal region containing zinc binding site of spire impair oocyte maturations and decrease cytoplasmic actin mesh. Taken together, these results suggest that intracellular zinc is crucial for the proper localizations of spire in the mouse oocyte, and unraveling the novel regulatory mode of actin nucleator spire by Zn2+.

키워드

Oocyte maturation Actin filament Spire Zinc

저자

  • Yu-Jin Jo [ Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • In-won Lee [ Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • Seong-min Jeong [ Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • Jeong-woo Kwon [ Department of Animal Science, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • Nam-Hyung Kim [ Department of Animal Science, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Cheongju 361-763, Republic of Korea ]
  • Suk Namgoong [ Department of Animal Science, Chungbuk National University, Cheongju 361-763, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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