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Melatonin Enhances the Developmental Competence of Porcine Embryos derived from Somatic Cell Nuclear Transfer by Preventing DNA Damage Induced by Oxidative Stress

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.94-94
  • 저자
    Shuang Liang, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347581

원문정보

초록

영어
Melatonin has antioxidant and scavenger effects in the cellular antioxidant system. It acts by protecting cells from oxidative injury via superoxide-free radicals and hydrogen peroxide (H2O2). Recent studies have shown that treatment with melatonin improves somatic cell nuclear transfer (SCNT) embryo development and increases the success of somatic cell reprogramming. However, the mechanisms for this remain unclear. This research investigated the protective effects and underlying mechanisms of action for melatonin in porcine SCNT embryos under oxidative stress. The results suggested that the developmental competence of porcine SCNT embryos was significantly enhanced after melatonin treatment. In addition, melatonin attenuated the H2O2-induced increase in reactive oxygen species levels, decrease in glutathione levels, and mitochondrial dysfunction. Importantly, melatonin also inhibited phospho-histone γH2A.X expression and comet tail formation, suggesting that it prevents H2O2-induced DNA damage. Meanwhile, the expression of genes involved in homologous recombination (MRE11a, BRAC1, and RAD51) and non-homologous end-joining (PRKDC, XRCC6, and TP53BP1) pathways for the repair of double-stranded breaks was reduced upon melatonin treatment in porcine SCNT embryos on day 5 of development under H2O2-induced oxidative stress. Taken together, these results indicated that melatonin promotes porcine SCNT embryo development by preventing oxidative stress-induced DNA damage via quenching of free radical formation. Our results reveal a previously unrecognized regulatory effect of melatonin in response to oxidative stress and DNA damage. This provides a novel mechanism for the improvements in SCNT embryo development that were reported to be associated with exposure to this hormone.

키워드

Melatonin Antioxidant DNA damage SCNT embryo development

저자

  • Shuang Liang [ Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, South Korea ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University, Cheongju 361-763, Republic of Korea, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, South Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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