Earticle

초록

영어

In mammals, cyclin-dependent kinases (CDKs) are involved in regulating both the cell cycle and transcription. Although CDK1 is known to act as the kinase subunit of maturation- promoting factor (MPF), the roles of the other CDKs in mammalian oocyte maturation are not yet understood. Here, we show that inhibition of various CDKs by small molecule inhibitors has different effects on the maturation and transcriptional activity of pig oocytes in vitro. Inhibition of CDK1 did not significantly affect cumulus cell expansion, but its kinase activity was necessary for germinal vesicle breakdown (GVBD). The inhibitions of CDK2, CDK4, or CDK6 had no effect on cumulus expansion or GVBD. The catalytic activity of CDK7 was crucial for GVBD but less important for cumulus expansion, whereas inhibition of CDK9 severely blocked both cumulus cell expansion and GVBD. CDK1, —2, —4, and —6 appeared to be dispensable for nuclear transcription, as their inhibitions did not affect nascent RNA production in oocytes. However, inhibition of CDK7 or CDK9 dramatically decreased the transcriptional activity in oocytes. Finally, we found that the GVBD arrest triggered by CDK9 inhibition was not due to altered MPF activity, but rather the inhibition of transcription. Overall, our results show that CDK7 and CDK9 are important for the nuclear maturation and transcriptional activity of pig oocytes.

키워드

저자

• Reza K. Oqani [ Department of Animal Science and Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 34134, Republic of Korea ]
• Tao Lin [ Department of Animal Science and Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 34134, Republic of Korea ]
• Jae Eun Lee [ Department of Animal Science and Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 34134, Republic of Korea ]
• So Yeon Kim [ Department of Animal Science and Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 34134, Republic of Korea ]
• Jung Won Kang [ Department of Animal Science and Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 34134, Republic of Korea ]
• Dong Il Jin [ Department of Animal Science and Biotechnology, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 34134, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636