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Poster Presentation : Oocyte Maturation / Embryonic Development

Supplement of Allicin during In Vitro Aging Enhances the Development of Parthenogenetic and Somatic Cell Nuclear Transfer Embryos

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.87-87
  • 저자
    Seung-Eun Lee, Yeo-Jin Son, Yun-Gwi Park, Hyuk Hyun, Hyo-Jin Park, Eun-Young Kim, Se-Pill Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347574

원문정보

초록

영어
Allicin (AL) is one of the chemical substance from garlic and has strong antioxidant activity and considered to represent anti-aging effect in vitro. The objective of this study was to investigate the effects of allicin treatment during porcine oocyte aging and their in vitro developmental competency after somatic cell nuclear transfer (SCNT). Porcine oocytes were maturated in vitro for 44 h (control), 44+24 h and 44+24 h with 0, 0.1, 1, 10 and 100 μM allicin (0, 0.1, 1, 10 and 100 AL, respectively). We investigated the effects of appropriate concentration of allicin on nuclear, cytoplasmic maturation and the developmental capacity of aged porcine oocytes. The 1 AL significantly increased maturation rate and normal spindle formation rate compared to control and 0 AL. The mRNA expression of cytoplasm maturation marker genes (MOS, BMP15, GDF9 and CCNB1) in 1 AL was higher than control and 0 AL. Allicin enhanced the rearrangement of abnormal spindle in aged oocytes. Parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryos development in 1 AL were significantly increased compared to others. Allicin treatment on aged oocytes improved their poor developmental capacity by rearranging the cytoskeleton. Thus, the allicin is effective agent to prevent the poor developmental competence of porcine aged oocytes and enhanced PA and SCNT embryo developmental capacity. Therefore, AL may be helpful for improve the aged oocytes quality to use on in vitro experiments.

키워드

Allicin PA SCNT Embryo development Aged oocyte

저자

  • Seung-Eun Lee [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Yeo-Jin Son [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Yun-Gwi Park [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Hyuk Hyun [ Jeju National University Stem Cell Research Center ]
  • Hyo-Jin Park [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Eun-Young Kim [ Jeju National University Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]
  • Se-Pill Park [ Jeju National University Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & Developmental Biology(Supplement) Volume 41 No 2 Supplement

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