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Poster Presentation : Oocyte Maturation / Embryonic Development

Optimization of Jeju Black Cattle IVF/IVC System

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.86-86
  • 저자
    Min-Young Shin, Seung-Eun Lee, Yeo-Jin Son, Dong-Yong Hong, Eun-Young Kim, Se-Pill Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347573

원문정보

초록

영어
Sperm treatment methods affect sperm fertility and in vitro developmental potential of in vitro fertilized (IVF) embryo. Jeju Black Cattle (JBC) is an indigenous species of Korea and IVF system is very important for mass production and industrialization of JBC. In order to find out the most suitable sperm treatment method, the following methods of percoll gradient, triladyl and percoll-triladyl combination (P, T and PT, respectively) were used to sperm treatment. At 2 days after IVF, the fertilization rate was higher in T and in PT groups than in P group. The blastocyst formation rate was significantly increased in T group than P and PT groups. The total cell number was higher in T and PT groups than in P group. The blastocyst formation rate was increased in T group. When we performed IVF after IVM treatment of individual ovaries, in comparison of the development rates of donor and non-donor cows, there was no significant difference. According to grade (1++, 1+, 1, 2), the development rate was the highest in 1++ grade cattle, followed by 1, 1+ and 2 grade. Also, in vitro developmental potential by carcass weight was the highest at the 300～350 kg, followed by 300 kg or less, 350～400 kg, then 400 kg more over. As a result, the most effective sperm processing was resulted in PT method, and the development of IVF embryos was more effective when the carcass grade was high and the carcass weight was about 350 kg. This study demonstrates that JBC sperm can be effectively recovered with simple and efficient PT method and from this protocol, high quality embryos can be produced in vitro.

키워드

Bovine Jeju black cattle Sperm treatment method In vitro fertilization Development

저자

  • Min-Young Shin [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Seung-Eun Lee [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Yeo-Jin Son [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Dong-Yong Hong [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Eun-Young Kim [ Jeju National University, Jeju National University Stem Cell Research Center, Mirae Cell Bio ]
  • Se-Pill Park [ Jeju National University, Jeju National University Stem Cell Research Center, Mirae Cell Bio ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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