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Poster Presentation : Oocyte Maturation / Embryonic Development

β-Cryptoxanthin Supplementation on In Vitro Maturation Medium Strengthens the Quality and Potential of Porcine Oocytes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.85-85
  • 저자
    Yun-Gwi Park, Seung-Eun Lee, Won-Jae Kim, Eun-Young Kim, Se-Pill Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347572

원문정보

초록

영어
One of the reasons about low quality of in vitro matured (IVM) oocytes than those of in vivo is the oxidative stress caused by external oxygen and incomplete antioxidant system. This study was performed to examine the effects of β-cryptoxanthin (BCX, anti- oxidative reagent) on porcine oocyte during IVM and further in vitro developmental potential. The oocytes were matured in IVM medium containing 0, 0.1, 1, 10 and 100 μM BCX (control, 0.1, 1, 10 and 100 B, respectively). The rate of oocyte maturation was higher in 1 B than in control (p<0.1), while that of other BCX treated groups were similar to control. After IVM, the cytoplasmic reactive oxygen species (ROS) expression level in 1 B was the lowest among all groups (p<0.05), while other BCX treated groups were similar to or higher than control. Also, at the classified oocyte maturation stages (GVBD, MⅠ and MⅡ), 1 B significantly decreased ROS and increased GSH level than control (p<0.05). In addition, the relative mRNA expression level of antioxidant genes, SOD1 and PRDX5, were higher in 1 B than in control (p<0.05). As the provitamin A, the relative mRNA expression level of retinoic acid receptor genes, ITGB7 and RXRA, were significantly increased in 1 B than in control (p<0.05). After parthenogenetic activation, the cleavage rates were not different between control and 1 B, however, the blastocyst formation rate was higher in 1 B than in control (p<0.01). In embryo quality, while the DNA fragmentation of blastocysts was similar between control and 1 B, the total cell number (p<0.1) and the relative mRNA expression level of pluripotency marker genes, Pou5f1 and CDX2 (p<0.05), and anti-apoptosis genes, Bcl2L1, Bcl-xl and BIRC5 (p<0.05), were significantly increased in 1 B than in control. These results demonstrate that BCX is helpful for decreasing oxidative stress in porcine oocytes and improves their quality and developmental potential.

키워드

β-cryptoxanthin Porcine Oocyte ROS Developmental potential

저자

  • Yun-Gwi Park [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Seung-Eun Lee [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Won-Jae Kim [ Jeju National University, Jeju National University Stem Cell Research Center ]
  • Eun-Young Kim [ Jeju National University, Jeju National University Stem Cell Research Center, Mirae Cell Bio ]
  • Se-Pill Park [ Jeju National University, Jeju National University Stem Cell Research Center, Mirae Cell Bio ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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