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Investigation of the Developmental Potential and Developmental Kinetics of Bovine Parthenogenetic and Somatic Cell Nuclear Transfer Embryos using a Time-Lapse Monitoring System

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.84-84
  • 저자
    Min-Jee Park, Eun-Young Kim, Se-Pill Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347571

원문정보

초록

영어
A time-lapse monitoring system has predictive value for selecting good-quality embryos with the highest implantation potential. Using this new tool, we investigated the developmental potential and developmental kinetics of bovine parthenogenetic (PA) and two types of somatic cell nuclear transfer (NT) embryos. Bovine non-transgenic ear cells (bECs) or transgenic cells (bTGCs) were used as donor cells. The cleavage and blastocyst development rates did not significantly differ among the PA, NT-bEC, and NT-bTGC groups, and first cleavage occurred an average of 19.3 h (n=70), 21.6 h (n=60), and 21.3 h (n=62) after activation, respectively (20.4 h (n=192) for all embryos). When embryos were classified into early cleaving (≤ 20 h) and late cleaving (> 20 h) groups, the blastocyst formation rate was much higher in the early cleaving groups (PA, 46%; NT-bEC, 50%; NT-bTGC, 39%) than in the late cleaving groups (PA, 18%; NT-bEC, 23%; NT-bTGC, 28%), while the percentage of embryos whose development was blocked between the 2- and 8-cell stages was increased in the late cleaving groups. The percentage of embryos classified as early cleaving with a normal morphology was 2-fold higher in the PA group (20.0%, n=14) than in the NT-bTGC group (9.7%, n=6). The timing of each developmental stage varied widely; the timing of first cleavage varied from 7.6 h in the PA group to 34.5 h in the NT-bEC group and the timing of expanded/hatching blastocyst appearance varied from 141.6 h in the PA group to 196.3 h in the NT-bTGC group, differences of 26.9 and 54.7 h, respectively (PA>NT-bEC>NT-bTGC). These results demonstrate that time-lapse monitoring provides novel data regarding individual embryo developmental kinetics and helps to predict developmental potential for improved bovine NT embryo selection based on early cleavage (< 20 h) and normal morphology.

키워드

Time-lapse Bovine embryos Transgenic

저자

  • Min-Jee Park [ Mirae Cell Bio, Jeju National University Stem Cell Research Center, Jeju National University ]
  • Eun-Young Kim [ Mirae Cell Bio, Jeju National University Stem Cell Research Center, Jeju National University ]
  • Se-Pill Park [ Mirae Cell Bio, Jeju National University Stem Cell Research Center, Jeju National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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