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Poster Presentation : Oocyte Maturation / Embryonic Development

Effect of Transmission of Exogenous Mitochondria into Bovine Parthenogenetic Embryos

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.83-83
  • 저자
    Young-Soo Cho, Sang-Ki Baek, Ik-Seong Kim, Joon-Hee Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347570

원문정보

초록

영어
Heterozygous status of recipient mitochondria and donor mitochondria in somatic cell nuclear transfer (SCNT) embryo is a quite defective. To solve the problem, our previous research has proposed that treatment of 2’,3’-dideoxycytidine (ddC) for in vitro maturation of oocyte induces the depletion of mtDNA in bovine oocytes. Bovine oocytes were matured in 20 μM ddC for 24h at 39℃ in a humidified atmosphere of 5% CO2. The second meiotic metaphase (MⅡ) oocytes were selected for oocyte activation and then activated by a combination of 5 μM inomycin for 5 min and 2 mM 6-DMAP for 3 h. GFP-tagged exogenous mitochondria were injected into the parthenogentic embryos using a 15 μM of injection pipette at 16 h after oocyte activation. Embryos were cultured in mSOFaa medium and changed medium every 2 days. At 7 days, the development rate of embyos was measured. GFP and hoechest were observed by a fluorescence microscope. As a result, the maturation rate of bovine oocytes were not significantly lower than that in the control oocyte. In addition, the development of embryos treated with 20 mM ddC for in vitro maturation and injected exogenous mitochondria was not different from that of the control. However, expression of GFP was showed only in the embryos injected exogenous mitochondria. In conclusion, we transmitted exogenous mitochondria into mtDNA depleted bovine oocytes.

키워드

Mitochondria 2’ 3’-dideoxycytidine Green fluorescent protein

저자

  • Young-Soo Cho [ Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea, Division of Applied Life Science (BK21 Program), IALS, PMBBRC, Gyeongsang National University, Jinju 52828, Republic of Korea ]
  • Sang-Ki Baek [ Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea, Division of Applied Life Science (BK21 Program), IALS, PMBBRC, Gyeongsang National University, Jinju 52828, Republic of Korea ]
  • Ik-Seong Kim [ Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea, Division of Applied Life Science (BK21 Program), IALS, PMBBRC, Gyeongsang National University, Jinju 52828, Republic of Korea ]
  • Joon-Hee Lee [ Department of Animal Bioscience, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea, Institute of Agriculture & Life Science, College of Agriculture & Life Sciences, Gyeongsang National University, Jinju 52828, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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