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Poster Presentation : Oocyte Maturation / Embryonic Development

The Role of PARylation in Porcine Cumulus-oocyte Complex during In Vitro Maturation

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.81-81
  • 저자
    Duk Hyoun Kim, Su Jin Jin, Min Gyeong Kim, Jun Sung Lee, Hoon Taek Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347568

원문정보

초록

영어
Poly(ADP-ribosyl)ation (PARylation) is related to DNA repair, chromatin modification, and apoptosis and catalyzed by poly (ADP-ribose) polymerases (PARPs). Recent studies have shown that PARylation affects preimplantation development and autophagy mechanism in pig embryos. However, the role of PARylation during in vitro maturation (IVM) has not yet been investigated. To study the effect of PARylation, cumulus-oocyte complexes were cultured with 3-aminobenzamide (3-ABA, PARP inhibitor) during IVM. Nuclear maturation of oocytes were showed no significant difference in all stages (GV, MI, MII), but full expansion of cumulus cell was significantly decreased (11.05±1.09 vs. 48.40±0.67%) in 3-ABA treatment group. To evaluate mRNA levels of maturation and expansion related genes, we analyzed 3-ABA treated oocytes and cumulus cells. GDF- 9, BMP15, COX-2, PTX3 expression were upregulated but levels of HAS2, TNFAIP6 were downregulated in treatment group. To evaluate the effect of PARP inhibitior on embryo development and blastocyst quality, we experimented parthenogenetic activation (PA). As a results, the developmental rate of PA embryos was significantly decreased in 3-ABA group. Furthermore, TUNEL index (4.80±0.66 vs. 2.42±0.35) and total apoptotic index (6.44±0.81 vs. 3.08±0.51) was significantly increased, while Total cell number was decreased (41.12±2.10 vs. 50.38±2.27). Therefore, these results suggested that PARylation plays an important role in IVM of porcine oocytes.

키워드

Cumulus expansion Poly(ADP-ribosyl)ation In vitro maturation

저자

  • Duk Hyoun Kim [ Department of Animal Biotechnology, Konkuk University ]
  • Su Jin Jin [ Department of Animal Biotechnology, Konkuk University ]
  • Min Gyeong Kim [ Department of Animal Biotechnology, Konkuk University ]
  • Jun Sung Lee [ Department of Animal Biotechnology, Konkuk University ]
  • Hoon Taek Lee [ Department of Animal Biotechnology, Konkuk University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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