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Poster Presentation : Oocyte Maturation / Embryonic Development

Sirtinol Treatment Influences Porcine Preimplatation Development Via Regulation of Autophagy and Apoptosis

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.80-80
  • 저자
    Min Gyeong Kim, Duk Hyoun Kim, Jun Sung Lee, Su Jin Jin, Hoon Taek Lee
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347567

원문정보

초록

영어
In vitro production of pig embryos has been emerged for xenotransplantation due to physiological properties. Sirtuins play a crucial role in several cellular processes including DNA repair, apoptosis, cell cycle, and determining lifespan as NAD+ dependent class III histone deacetylase. Previous studies have shown that sirtuin inhibition induces embryonic developmental arrest and oxidative stress in porcine and murine models. Also, sirtuins regulate autophagy pathways through the deacetylation of ATG proteins and transcriptional factors. However, sirtuins-mediated mechanisms have not been examined in blastocysts. Therefore, we investigated the relationship between sirtuin inhibition and autophagy in porcine model. Embryos were cultured in 100 μM sirtinol (SIRT1/2 target inhibitor)-treated NCSU-23 media after in vitro fertilization. Treatment with sirtinol significantly decreased the rates of morula (21.34±1.84 vs. 11.89±2.01%), blastocyst formation (17.18±1.81 vs. 9.00±2.02%), and total cell number (50.80±1.47 vs. 37.71±1.79). Especially, expanded blastocysts (9.90±1.56 vs. 2.92±0.94%) were barely observed in sirtinol treated group. The level of Sirt2 expression was significantly decreased in sirtinol-treated group compared to control. Also, sirtinol induced autophagy by increasing LC3 transcript and LC3 protein in treatment group. Beclin1 and ATG5 expressions showed a slight increase in sirtinol-treated group. Finally, sirtuin inhibition by sirtinol showed significantly increased TUNEL indices (6.55±0.84 vs. 11.44±0.81) and fragmentation indices (0.33±0.05 vs. 1.40±0.30). Similarly, the level of BCL2L1 was lower, while the level of Caspase-3 expression was significantly elevated in the treated group compared to controls. Therefore, these results suggest that sirtuin inhibition may play an important role in porcine preimplantation development and embryo quality through regulation of autophagy and apoptosis pathways.

키워드

Sirtuin Autophagy Apoptosis Porcine blastocyst

저자

  • Min Gyeong Kim [ Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul 05029, South Korea ]
  • Duk Hyoun Kim [ Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul 05029, South Korea ]
  • Jun Sung Lee [ Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul 05029, South Korea ]
  • Su Jin Jin [ Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul 05029, South Korea ]
  • Hoon Taek Lee [ Department of Animal Biotechnology, Bio-Organ Research Center, Konkuk University, Seoul 05029, South Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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