Earticle

초록

영어

Plasminogen activators system (PAs) is one of important physiological system for reproductive events such as oocyte maturation, acrosome reaction, fertilization, and uterine remodeling. It is consist of two-types of PAs (urokinase-type, uPA; tissue-type, tPA), uPA-specific receptor (uPAR), and type-1 PA inhibitor (PAI-1). In various type of cells, activity of these system was regulated by gonadotropin, cytokines, and hormones, however, regulation of mRNA expression in porcine uterine cells was not clear. Therefore, this study aimed to investigate the effect of progesterone (P4) on mRNA expression of uPA, tPA, uPAR, and PAI-1 in epithelial cells of porcine uterine endometrium. Epithelial cells were isolated from luminal epithelium in endometrium and cultured at 80% confluence. Then, it were subsequently incubated with different concentration of P4 (0, 0.1, 1, 5, 10, and 20 ng/mL) for 5 min and 24 h at 38.5℃, 5% CO2 in air. mRNA expression was measured by reverse-transcription PCR method. In results, expression of uPA mRNA in P4-exposed epithelial cell for 5 min was increased by 0.1 and 20 ng/mL P4 treatment. However, 1, 5, and 10 ng/mL P4 reduced uPA mRNA expression compared to 0.1 ng/mL treatment (p<0.05). On the other hand, all P4 treatment groups for 24 h enhanced mRNA expression of uPA. tPA mRNA in cultrued cells with P4 for 5 min and 24 h was enhanced by 10 and 20 ng/mL treatment, whereas 5 ng/mL P4 reduced. And 20 ng/mL P4 treatment increased uPAR mRNA expression in epithelial cells that were incubated for 5 min and 24 h. Similar to result of tPA mRNA, PAI-1 expression was enhanced by 5, 10, and 20 ng/mL treatment groups. The findings in this study show that mRNA expression of PAs system components were differently regulated by P4 in porcine uterine cells and further study regarding to regulation of this system was needed.

키워드

저자

• Yong Hwang Bo [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
• Hee-Tae Cheong [ School of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
• Boo-Keun Yang [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
• Choon-Keun Park [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636