Earticle

초록

영어

Progesterone primes the endometrium for possible implantation and inhibits uterine contractions until birth. 20α-Hydroxysteroid dehydrogenase (20α-HSD) is a member of the aldo-keto reductase (AKR) family. These enzymes play pivotal roles in the modulation and regulation of steroid hormones, such as androgens, estrogens, and progestins, and are thus considered important targets for drug design. In the present study, we investigated the expression pattern of 20α-HSD during pregnancy in bovine luteal tissues. And, the amounts of mRNA were determined in cultured cells of the corpus luteum cells(CL). The localization of 20α-HSD was also determined in luteum during early pregnancy. Analysis of expression pattern of progesterone inhibitor gene 20a-HSD and apoptosis mechanism in the culture cell. Collected of during cells were the cultured under 20a-HSD(0.375 ug/mL(type 1), 0.75 ug/mL(type 2), and 1.125 ug/mL(type 3) in 48 hr. and to determine the function of 20α-HSD gene during pregnancy, we collected the pregnant corpus luteal on 30, 60 and 90 days of pregnancy. The first results found that the level of 20α-HSD was the most highly expressed in the corpus luteal day 90. And the luteum progressively increased from day 30, 60 to 90. Furthermore apoptotic detected was the most highly expressed in the corpus luteal day 30 and 90. The secondly results, that the type 1 cultured, 20α-HSD and Casp-3 protein was the most highly expressed throughout cytoplasm. However, decreased 20α-HSD expression on the type 3. Interestingly, 20α-HSD and Casp-3 proteins was lowest expressed in type 2, cytosol is showing levels higher than cytoplasm. These results suggest that the 20α-HSD plays a role in maintaining normal pregnancy at least partially by controlling the progesterone hormone concentrations during development of copus luteum cell.

키워드

저자

• Ji-Yeon Kim [ Department of Animal Life Research, Hankyong National University ]
• Sang-Hwan Kim [ Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea ]
• Enkholor [ Major in the Animal Biotechnology The Graduate School of Biology & Information Technology, Hankyong National University, Anseong 456-749, Korea ]
• Ji-Hye Lee [ Major in the Animal Biotechnology The Graduate School of Biology & Information Technology, Hankyong National University, Anseong 456-749, Korea ]
• Jong-Taek Yoon [ Department of Animal Life Research, Hankyong National University, Institute of Genetic Engineering, Hankyong National University, Ansung 456-749, Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636