Earticle

초록

영어

Gonadotropin receptors are members of the seven transmembrane receptor families. Several point mutations in the third intracellular loop have been identified in the follicle stimulating hormone receptor (FSHR) gene leading to constitutive activation and inactivation of the receptor. The FSHR gene mutations cause a form of ovarian resistance (savage syndrome) in females and small testes and abnormal sperm parameters in males. In eelFSHR, we generated 1 type of constitutive activating mutation (D566G) and 2 types of constitutive inactivating mutations (A189V, N191I) to investigate how they work on hormone-receptor interaction and receptor activation system on eel rec- FSH. To assess the functional effects of 3 receptor mutations directly, wild type and mutant eelFSHRs were constructed to pVSV-G expression vector and transiently transfected to CHO-K1 cells with expressing vector cloned in pVSVG (6 well/plate; 2,500 ng). And then cAMP accumulation by rec-eelFSH stimulated was measured. Rec-eel- FSH produced a concentration-dependent increase in cAMP production in wild-type receptor. Next, we transfected with D566G plasmid (6 well/plate; 5,000 ng). It was drastically elevated basal cAMP level compared to wild-type. The basal cAMP levels were 11.5 nM and 25.8 nM, respectively. Thus, D556G in the basal cAMP responsiveness was 2.2 times than that of wild type. However, the inactivation mutant A189V did not obviously altered. In summary, we suggest that mutation of D566G is responsible for constitutive activation in eel FSHR like mammalian FSHR.

키워드

저자

• Jeong-Soo Kim [ Department of Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Ansung-si 456-749, South Korea ]
• Munkhzaya Byambaragchaa [ Department of Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Ansung-si 456-749, South Korea ]
• Hun Ki Seong [ Department of Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Ansung-si 456-749, South Korea ]
• Kwan-Sik Min [ Department of Animal Biotechnology, Graduate School of Future Convergence Technology, Hankyong National University, Ansung-si 456-749, South Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636