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Poster Presentation : Gene Expression / Function

Roles of CPEB2 by Regulating of Tight Junction Biogenesis during Porcine Early Embryo Development

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.53-53
  • 저자
    Sun-ha Park, Jeongwoo Kwon, Min-Jung Seong, In-chul Choi, Nam-Hyung Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347551

원문정보

초록

영어
CPEB-mediated tight junction assembly was identified in mammary epithelial cell polarity but not known in early embryos. The objective of this study is to determine expression and function of CPEB2 during blastocyst formation in porcine parthenote developing. Transcriptional level of CPEB was identified by qPCR, and protein expression was observed by immunocytochemistry. Double strand RNA(dsRNA) of CPEB2 was injected into zygotes to identify and cultured in vitro to identify the function of CPEB2. The formation of tight junction protein was confirmed by FITC dextran assay. The overall localization of CPEB3 and cell junction related proteins (ZO-1, CAR, Occludin, E-cadherin, ß-catenin) were also confirmed in CPEB2 knock downed embryos. CPEB2 proteins were identified in nucleus until 8-cell stage, but localized in cytoplasm in morula and blastocyst embryos. Knock down of CPEB2 using double strand RNA reduced development to blastocysts and blastocyst size. FITC-dextran assay showed that GFP-dextran positive embryo was increased in the KD (28.3%) than the control (5.7%). ZO-1, CAR, and OCLN, was mislocalized in CPEB2 KD embryos. These results suggest that the formation of tight junction protein by CPEB2 plays a crucial role in embryonic development in mammals.

키워드

CPEB2 Tight junction biogenesis Embryo development Polyadenylation

저자

  • Sun-ha Park [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, South Korea ]
  • Jeongwoo Kwon [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, South Korea ]
  • Min-Jung Seong [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, South Korea ]
  • In-chul Choi [ Department of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University, Korea ]
  • Nam-Hyung Kim [ Department of Animal Sciences, Chungbuk National University, Brain Korea 21 Center for Bio-Resource Development, Chungbuk National University, South Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & Developmental Biology(Supplement) Volume 41 No 2 Supplement

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