Earticle

현재 위치 Home

Poster Presentation : Gene Expression / Function

Isolation and Characterization of Spermatogonial Stem Cells in Chicken

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.48-48
  • 저자
    Hoonsung Choi, Sun Keun Jung, Jeom Sun Kim, Kyung-Woon Kim, Keon Bong Oh, Hyeon Yang, Dong-Hoon Kim, Sung June Byun
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347546

원문정보

초록

영어
Spermatogonial stem cells (SSCs) are expected to undergo self-renewal and differentiation into different body cell types in vitro, which are expected to serve as a powerful tool and resource for transgenic animal technology. We have established an efficient isolating for SSCs from 1-day-old chicken testicular cells. This present study is aimed to carry out the culture and characterization of chicken SSCs. Disassociation of chicken SSCs were performed using two-step enzymatic digestion of type 4 collagenase and trypsin and were seeded in a twelve-well plate. In primary culture was observed colony forming on day four post incubation. In the subsequent passages, chicken SSCs were positive for alkaline phosphatase activity and showed positive expression for the transcription factor, Oct 4. Immunoblotting analysis revealed that the chicken SSCs showed expression for the GRP78. Therefore, our findings may have implications for developmental biology and regenerative medicine by using chicken as the transgenic animal model.

키워드

Chicken embryo Spermatogonial stem cells

저자

  • Hoonsung Choi [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Sun Keun Jung [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Jeom Sun Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Kyung-Woon Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Hyeon Yang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Dong-Hoon Kim [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Sung June Byun [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / Reproductive & Developmental Biology(Supplement) Volume 41 No 2 Supplement

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.