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Poster Presentation : Gene Expression / Function

In Vitro Culture of Rat Primary Hepatocytes Employing Monolayer and Spheroid Culture Environments

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  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.46-46
  • 저자
    Imran Ullah, Yeongji Kim, Malgum Lim, Keon Bong Oh, Seongsoo Hwang, Yurianna Shin, Youngim Kim, Gi-Sun Im, Tai-Young Hur, Sun A Ock
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347544

원문정보

초록

영어
Primary hepatocytes (PH) are considered as “gold standard” for drug screening because of its ability to express the entire set of drug metabolizing enzymes and transporters. Hepatocytes culturing and maintaining their hepatocyte fate in vitro is one of the major issue from last decade. The main problem in hepatocytes in vitro culturing is that, these cells rapidly loss their hepatic morphology and liver specific function in culture condition. In the present study, we isolated rat PH and cultured in monolayer (2D) as well as spheroid (3D) culture system. The 2D cultured PH hepatocyte showed an elongated hepatocyte morphology while, 3D cultured PH showed spheroid morphology with gradual decrease in diameter up to 7 days. After 7 days of in vitro culture, these cells were analyzed for the expression of hepatic markers (Alb, Tf, Afp) and apoptotic markers (Bax, Bcl2). Furthermore, PH in both culture systems was induced with two inducers i.e. 3-methylcholanthrene (3-MC, Cyp1a specific inducer) and dexamethasone (Cyp3a specific inducer) for 48 and 72 hours, respectively. The mRNA level of Cyp1a and Cyp3a were analyzed in induced (3-MC, dexamethasone) and non-induced PH, respectively. After 7 days in vitro culture, PH showed dramatic down regulation of hepatic markers in both culture systems. Furthermore, expression of apoptotic markers was higher in 2D cultured PH as compared to 3D. Cyp1a and Cyp3a mRNA level showed higher RNA content in 2D culture PH after 48 of induction. Therefore, we concluded that there was no significant difference found in two culture system and further studies are needed to find out the essential components for PH in vitro culture rather than culture system.

키워드

Rat Primary hepatocytes Spheroid (3D) culture CYP1A CYP3A

저자

  • Imran Ullah [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Yeongji Kim [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Malgum Lim [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Keon Bong Oh [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Seongsoo Hwang [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Yurianna Shin [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Youngim Kim [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Gi-Sun Im [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Tai-Young Hur [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]
  • Sun A Ock [ Division of Biotechnology, National Institute of Animal Science, Rural Development Administration, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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