Earticle

초록

영어

Spermatogenesis is the process of proliferation and differentiation of spermatogonial stem cells into adult spermatozoa within seminiferous tubules. Sperm cells undergo extensive epigenetic modifications during spermatogenesis. One of the epigenetic modifications, histone acetylation induces a loose chromatin structure and active transcription which facilitates binding of transcription factors at sperm chromosome. Therefore, histone acetylation is known to play an important role in spermatogenesis. However, factors involved in histone acetylation during spermatogenesis are not well known. We found a factor TLE3(Transducin Like Enhancer of Split 3). The transcriptional co-repressor TLE family is known to regulate histone acetylation by interacting with histone deacetylase( HDAC). We examined the expression level of TLE3 in various mouse tissues. As a result of RT-PCR, TLE3 showed significantly higher expression in testis than that in other tissues. Immunofluorescent and Immunohistochemical analysis revealed that TLE3 protein was located in sertoli cell and spermatid in seminiferous tubules of adult mouse testis. In addition, the differential regulation in TLE3 protein expression site was observed in the testis during postnatal development. We also analyzed the expression pattern of HDAC2, which is known to cooperate with TLE3. Both HDAC2 and TLE3 protein were detected in spermatogonial stem cells at one-weeks-old mouse, however, Their expression site were transferred to sertoli cell at six-weeks-old mouse. aken together, TLE3 may play a role in regulating histone acetylation via interaction with HDAC2 in the testis. Futher studies are needed to look into a relation between TLE3 and histone acetylation during spermatogenesis and postnatal development in testis.

키워드

저자

• Sangho Lee [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Sohyeon Moon [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Minha Cho [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Miseon Park [ Fertility Center of CHA Gangnam Medical Center, Seoul, Republic of Korea ]
• Boreum Song [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Ok-Hee Lee [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Hoon Jang [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Kyung-Ah Lee [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Sunghan Shim [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea, Fertility Center of CHA Gangnam Medical Center, Seoul, Republic of Korea ]
• Jung Jae Ko [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea ]
• Youngsok Choi [ Department of Biomedical Science, CHA University, Seoul, Republic of Korea, Fertility Center of CHA Gangnam Medical Center, Seoul, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  Reproductive & Developmental Biology(Supplement)

• 간기

  연간

• 수록기간

  2001~2017

• 십진분류

  KDC 527 DDC 636