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Screening of Integrins in Porcine Embryonic Stem-like Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    Reproductive & Developmental Biology(Supplement) 바로가기
  • 통권
    Volume 41 No 2 Supplement (2017.06)바로가기
  • 페이지
    pp.13-13
  • 저자
    Hwa-Young Kim, Na Rae Han, Song Baek, Seung Tae Lee, Eun Song Lee, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347522

원문정보

초록

영어
Porcine embryonic stem-like cells (pESLCs) are able to undifferentiated proliferation and self renewal. However, due to the lack of a system that can effectively culture pESLCs, it is difficult to long-term culture of pESLCs. Integrins composed of α and β subunits and αβ combination are molecular receptor acting on the cell surface when cells are bound to extracellular matrix such as fibronectin and collagen. Integrins regulate function of cells through the inside-out and outside-in signaling regarding motility, survival, differentiation and proliferation of cells. However, there is lack of information on integrins regarding to pESLCs. Therefore, the aim of this study was to confirm the presence of integrins in pESLCs through the integrins screening. Fluorescence immuno assay showed that integrin α1 and α2 were not expressed, whereas, integrin α3, α4, α5, α6, α7, α8, α9, αv and β1 were expressed in pESLCs. Also, fluorescence intensity showed that intensity of integrin α5, α7, and α8 was higher than twice compared to other groups (610.58±26.83; 419.69±13.84; 646.09±4.53; p<0.05), but, integrin α6 was lower intensity than other groups (50.55±2.65; p<0.05). To identify of integrin heteromiders subsequently, we experimented the cell attachment assay. Cultures with 10 μg/mL vitronectin and 60 μg/mL tenascin C showed significantly highest rate compared to controls, indicating the presence of integrin heterodimer α9β1 and αvβ1 on the membranes of pESLCs respectively (p<0.05). Therefore, integrin α3, α4, α5, α6 and α7 exist as subunits and integrin α9β1 and αvβ1 exist as heterodimers in pESLCs.

키워드

Embryonic stem-like cells Extracellular matrix Integrin Pig

저자

  • Hwa-Young Kim [ College of Animal Life Science Kangwon National University, South Korea ]
  • Na Rae Han [ College of Animal Life Science Kangwon National University, South Korea ]
  • Song Baek [ College of Animal Life Science Kangwon National University, South Korea ]
  • Seung Tae Lee [ College of Animal Life Science Kangwon National University, South Korea ]
  • Eun Song Lee [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Republic of Korea ]
  • Choon-Keun Park [ College of Animal Life Science Kangwon National University, South Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    Reproductive & Developmental Biology(Supplement)
  • 간기
    연간
  • 수록기간
    2001~2017
  • 십진분류
    KDC 527 DDC 636

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