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Poster Presentation : Transgenesis / Xenotransplatation

Cytotoxicity Assay of US11 & hDAF Genes, and Natural Breeding Effect in Transgenic Pigs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.140-140
  • 저자
    Hyeon Yeong Shin, Tao Lin, Jung Won Kang, Jae Eun Lee, Dong Il Jin
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347513

원문정보

초록

영어
Unlike allograft, xenotransplantation has problems such as hyperacute rejection (HAR), acute rejection and chronic rejection. HAR was rapid humoral immune responses against the pig carbohydrate Galα1,3-Gal epitope by human complement regulatory reaction, but HAR was almost overcome through knockout of α1,3-galactosyltransferase gene. The acute rejection is appeared within a few days to week caused by ischemia, diffuse intravascular coagulation and lack of cellular infiltrate. In this study, we bred the transgenic pigs containing US11 and hDAF (CD55) gene, evaluate transgene transmission ratio and efficiency of Reproduction rate in natural breeding. In addition, performed Cytotoxicity experiment about human NK cells and complement against each piglet ear fibroblast that have gene expression of US11, hDAF or both hDAF and US11. In breeding evaluate, sow has normal litter size, and piglets sex rate was followed 1:1. Transmission ratio of transgenes was indicated that wild type was 10%, US11 gene only was 20%, hDAF gene only was 20%, and both US11 gene and hDAF gene was 50% in 10 piglets. In cytotoxicity assay, US11 gene showed that effect to reduce cytotoxicity rate from human NK cell, and hDAF gene was significantly reduce the cytotoxicity ratio from complement. Besides, piglet cells that has both US11 gene and hDAF gene has effect to reduce cytotoxicity of NK cell and complement. In conclusion, our study showed that US11 and hDAF genes has effective to reduced Xenotransplantation rejection, and natural breeding was efficient to product transgenic pig.

키워드

Cytotoxicity US11 hDAF Xenotransplantation

저자

  • Hyeon Yeong Shin [ Department of Animal Science & Biotechnology, Chungnam National University ]
  • Tao Lin [ Department of Animal Science & Biotechnology, Chungnam National University ]
  • Jung Won Kang [ Department of Animal Science & Biotechnology, Chungnam National University ]
  • Jae Eun Lee [ Department of Animal Science & Biotechnology, Chungnam National University ]
  • Dong Il Jin [ Department of Animal Science & Biotechnology, Chungnam National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회

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