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Poster Presentation : Stem Cells

Establishment of Intermediated Stem-like Cell from Mouse Skin Fibroblasts by Bone Morphogenetic Protein 4 and Small Molecules

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.134-134
  • 저자
    Seung-Eun Lee, Yun-Gwi Park, Eun-Young Kim, Se-Pill Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347510

원문정보

초록

영어
To establish induced pluripotent stem cell (iPSC) by reprogramming cells that is differentiated types, specific transcription factors are needed. Some researchers examined whether chemical molecules or proteins can replace transcription factors to alter the de fined cell fate. Previously, we confirmed that the treatment of bone morphogenetic protein 4 (BMP4) could intermediately reprogram the mouse skin fibroblasts (MSFs) to stem-like cells. In this study, to reprogram the cell more efficiently and definitely, we investigated the synergic effects of BMP4 and 4 kinds of small molecules, RG108, 5-azacytidine, Na-butyrate and PD173074, that regulates some signal transduction pathway. The ventral skin epidermis of an adult mouse were used to confirm the synergic reprogramming activity of small molecules. After the cells were cultured with BMP4, they were formed sphere-like organoid bodies (OBs) with BMP4 and each small molecules for 5 days. The activity of alkaline phosphatase was the highest in BMP4+RG108 group. Also, all the small molecule treated groups expressed high Oct-4 level than BMP4 treated group. To confirm the stem cell potency of these cells, we induced their differentiation into cardiomyocytes. The cells exhibited similar mRNA expression of cardiac mesoderm markers such as connexin 40 and GATA binding protein 4, and the cardiomyocyte marker troponin T among groups. However, the expression of one of the cardiac mesoderm marker gene, Nk2 transcription factor-related locus 5, was significantly decreased in BMP4+RG108 group. These results suggest that treatment of some small molecules may enhance the BMP4-mediated somatic stem cell reprogramming.

키워드

BMP4 Small molecule Cell reprogramming

저자

  • Seung-Eun Lee [ Jeju National University Stem Cell Research Center, Jeju National University ]
  • Yun-Gwi Park [ Jeju National University Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]
  • Eun-Young Kim [ Jeju National University Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]
  • Se-Pill Park [ Jeju National University Stem Cell Research Center, Jeju National University, Mirae Cell Bio ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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