Earticle

초록

영어

Mammalian testes are maintained at a lower temperature than the abdominal region for healthy spermatogenesis. Germ cell apoptosis has been described in heat-damaged testes by cryptorchidism, but the mechanism is not yet fully understood. To elucidate the cause of germ-cell death by cryptorchidism, we surgically induced cryptorchidism in dog testes and performed histological and molecular analyses. Histological data showed that the seminiferous tubules of cryptorchid testes and epididymis contained significantly fewer germ cells. Total RNA sequencing was performed to screen overexpressed genes in cryptorchid dog testes. Clusterin RNA was significantly higher (approximately 12.8-fold) in cryptorchid testes than in normal testes. In addition, cleaved caspase-3 and -8 were detected at higher levels in cryptorchid dog testes. Real time RT-PCR and western blotting analysis showed that the expression of clusterin was higher in cryptorchid dog testes. Furthermore, clusterin was detected in extracellular regions of cryptorchid dog testes during 4 weeks after surgery. Thus, we demonstrated germ-cell specific apoptosis and expression of clusterin in extracellular regions of cryptorchid dog testes. This result will facilitate further study of spermatogenesis and the specific mechanisms by which cryptorchidism causes male infertility.

키워드

저자

• Won-Young Lee [ Department of Beef & Dairy Science, Korea National College of Agricultures and Fisheries, Jeonbuk 54874, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636