Sung Woo Kim, Jinwook Lee, Chan-Lan Kim, Kwan Woo Kim, Yeong Gyu Ko, Ik Soo Jeon
언어
영어(ENG)
URL
https://www.earticle.net/Article/A347502
원문정보
초록
영어
This study examined factors affecting survival rate of epididymal sperm from Korean native black goat using 0.25 mL straw. The sperm was cryopreserved by the height above 5 cm or 14 cm from the surface of liquid nitrogen (LN2) for 10 min and then plunged into the LN2. The motility of sperm after thawing was estimated using CASA system and movement analysis. The motile sperm rates after thawing at 14 cm freezing height was significantly higher than at 5 cm (85.3% vs. 73.0%). Also the hyper- activation rates showed that the more actively moving sperm counts appeared at 14 cm height experiment (55.3% vs. 47.6%). This phenomenon was also observed in the dilution of electro-ejaculted semen. So, these results show that testis originated sperm could be used for artificial insemination and fertility evaluation in vivo is needed to evaluate their contribution to animal genetic resource banks.
키워드
Korean black goat epididymal spermCryopreservation
저자
Sung Woo Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
Jinwook Lee [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
Chan-Lan Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
Kwan Woo Kim [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
Yeong Gyu Ko [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]
Ik Soo Jeon [ Animal Genetic Resources Research Center, National Institute of Animal Science, RDA, Namwon 55717, Korea ]