Sang-Rae Cho, Sung-Sik Kang, Ui-Hyung Kim, Seok-Dong Lee, Myung-Suk Lee
언어
영어(ENG)
URL
https://www.earticle.net/Article/A347501
원문정보
초록
영어
The aim of the present study was investigated to determine optimal culture condition for IVP embryos using oocytes derived from follicles of ovum pick-up (OPU). Follicular oocytes were aspirated from Hanwoo native cows by the OPU method. After follicular aspiration, the number and morphology of cumulus-oocytes complexes (COCs) and the developmental competence of oocytes after in vitro maturation (IVM), in vitro fertilization (IVF) and in vitro culture were evaluated. The quality of aspirated COCs did not differ among the individual donor. The rates of oocytes recovery from the follicles by ultasound-guided aspiration were not significantly different among individual, but the rate of oocytes useful for IVF was significantly higher (p<0.05) in cows (67%) than heifers (45%) The cleavage and the developmental rate to blastocysts was not significantly different between CR1aa and IVMD medium. In conclusion, the culture medium did not have any apparent effects on the quality of aspirated COCs and developmental competence of oocytes after IVM-IVF, but it may affect follicular recruitment in non-pregnant cows. Moreover, the reproductive phase may influence the developmental competence of the recovered oocytes.
키워드
HanwooEmbryoOvum Pck-Up (OPU)
저자
Sang-Rae Cho [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Republic of Korea ]
Sung-Sik Kang [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Republic of Korea ]
Ui-Hyung Kim [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Republic of Korea ]
Seok-Dong Lee [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Republic of Korea ]
Myung-Suk Lee [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang 25340, Republic of Korea ]