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Poster Presentation : Sperm / Male Reproduction

Comparison of Two Cauda Epididymal Sperm Recovery Methods in Hanwoo Bulls by Flushing and Floating Techniques

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.119-119
  • 저자
    Sung-Sik Kang, Sang-Rae Cho, Ui-Hyung Kim, Seok-Dong Lee, Myung-Suk Lee, Boh-Suk Yang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347499

원문정보

초록

영어
In the present study, we compared cauda epididymal sperm recovery methods in Hanwoo bulls by flushing and floating techniques. Fourteen testicles with epididymides were collected from 7 Hanwoo bulls (months of age=15.1±0.2, scrotal circumference (cm)=31.6±1.1, weight (kg)=391.1±16.6. Epididymis with vas deferens in each bull was isolated from testis and blood vessels on surface of cauda epididymis. To recover cauda epididymal sperm by flushing and floating, each testis was randomly selected from a pair of testicles. The mean weight, length, width, circumference of testicles were similar. For flushing method, a 26 gauge needle connected with 10 mL syringe was inserted into the duct of vas deference. About 12 to 15 incisions were made in cauda epididymis. Pre-warmed semen freezing medium (OptixCell, IMV, France) flushed and epididymal sperm were recovered. For floating method, cauda epididymis was cut and minced using blades in a 100 mm dish. Minced cauda epididymis tissues were incubated with 10 mL of semen freezing medium for 15 min at room temperature. Sperm suspension recovered by filteration with a cell strainer (100 μM nylon mesh). Sperm concentration adjusted to 40 to 50×106 cells/mL with semen freezing medium, semen dilution loaded to 0.5 mL straw and cryopreserved in liquid nitrogen tank. Frozen-semen was thawed at 37°C for 40 sec and measured sperm motility and motility parameters using a CASA system (sperm class analyzer, Spain). Five replicates were conducted in each experimental group of bull. Recovered semen volume (63.3±28.0 vs. 61.7±23.0 mL), motility (89.5±12.8 vs. 91.4±7.9%) among flushing and floating methods were not significantly different. In conclusion, the both of flushing and floating techniques can be used for cauda epididymal sperm recovery without difference of sperm quality.

키워드

Cauda epididymal sperm Hanwoo Recovery method

저자

  • Sung-Sik Kang [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang, Kangwondo 25340, Republic of Korea ]
  • Sang-Rae Cho [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang, Kangwondo 25340, Republic of Korea ]
  • Ui-Hyung Kim [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang, Kangwondo 25340, Republic of Korea ]
  • Seok-Dong Lee [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang, Kangwondo 25340, Republic of Korea ]
  • Myung-Suk Lee [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang, Kangwondo 25340, Republic of Korea ]
  • Boh-Suk Yang [ Hanwoo Research Institute, NIAS, RDA, Pyeongchang, Kangwondo 25340, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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