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Poster Presentation : Sperm / Male Reproduction

Effects of Temephos on Sperm Function

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.114-114
  • 저자
    So-Hye Kim, Jeong-Won Bae, Woo-Sung Choi, Hong-Joon Choi, Woo-Sung Kwon
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347494

원문정보

초록

영어
Temephos is recommended a non-systemic organophosphorus insecticide by WHO because it is safe for humans and has low toxicity to mammals compared with other chemical compounds such as chlorine, potassium permanganate, zinc carbamate, and dichlorodiphenyltrichloroethane (DDT). However, Temephos is involved in the hazardous pesticide category PAN Bad Actor (Pesticide Action Network, USA). Moreover, no study was performed to evaluate toxicity of Temephos in mammalian germ cell. The objectives of this study was to assess the effect of Temephos on sperm functions. First, caudal epididymis spermatozoa from ICR mice were incubated with Temephos (0, 0.1, 1, 10, and 100 μM). Then sperm motility and motion kinematics were assessed using computer-assisted sperm analysis. In addition, capacitation status and adenosine triphosphate (ATP) level were assessed using combined Hoechst 33258/chlortetracycline fluorescence and ATP detection kit, respectively. The acrosome reaction and capacitation status were significantly increased and decreased in the highest concentration of Temephos (100 μM), respectively. In addition, ATP levels in spermatozoa were significantly decreased in higher concentration of Temephos (1, 10, and 100 μM). However, Sperm motility and motion kinematics (motility, medium, slow, progressive, hyperactivated, curvilinear velocity, straight-line velocity, average path velocity, beatcross frequency, dance mean, amplitude of lateral head displacement) were not significantly altered with Temephos. The results were shown that Temephos affect capacitation status, the acrosome reaction and ATP level. Taken together, we suggest that the present study may be applied to evaluate risk of Temephos on male fertility.

키워드

Temephos Capacitaition Acrosome reaction Adenosine triphosphate Spermatozoa

저자

  • So-Hye Kim [ Department of Animal Biotechnology, Kyungpook National University ]
  • Jeong-Won Bae [ Department of Animal Biotechnology, Kyungpook National University ]
  • Woo-Sung Choi [ Department of Animal Biotechnology, Kyungpook National University ]
  • Hong-Joon Choi [ Department of Animal Biotechnology, Kyungpook National University ]
  • Woo-Sung Kwon [ Department of Animal Biotechnology, Kyungpook National University ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회

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