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Poster Presentation : Sperm / Male Reproduction

Changes of Characteristics of Frozen-thawed Boar Sperm by Supplementation of Alpha-lonolenic Acid with Carrier Protein

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.112-112
  • 저자
    Won-Hee Lee, Wook-Hwan Kim, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347492

원문정보

초록

영어
As an one of detrimental effect during freezing and thawing process, fatty acids in plasma membrane of spermatozoa were released and sperm membrane was damaged. And several studies had reported that spermatozoa could absorb fatty acid in freezing extender. We expected that supplement of alpha-linolenic acid (ALA) during freezing process could improved efficiency of cryopreservation for boar sperm. Therefore, the aim of this study was to evaluate effect of ALA combined with Bovine Serum Albumin (BSA) and Methyl-β-Cyclodextrin (MβCD) on viability, acrosome reaction and mitochondrial intact in frozen-thawed boar sperm. For preparation of ALA-carrier protein complex, 3 ng/mL ALA was mixed with 0.7 μg/mL Bovine Serum Albumin (BSA) or 14 ng/mL Methyl-β-Cyclodextrin (MβCD) in distilled water. The boar semen was purchased from GUMBO Company. Boar semen was cryo-preserved in lactose-egg yolk (LEY) containing ALA, BSA, MβCD, ALA+BSA, ALA+MβCD and frozen sperm was thawed at 37.5℃ for 45 sec in water-bath. Sperm viability, acrosome reaction, and mitochondrial intact were analyzed using flow cytometry. In results, viability of frozen-thawed sperm was increased in all treatment groups. However, there was no significant difference. Acrosome reaction and mitochondrial damage in frozen-thawed sperm was decreased in all treatment groups compared with control group. However, there was no significant difference. In all sperm characteristics, ALA+BSA treatment group was higher than all treatment groups. In conclusion, addition of ALA with carrier proteins during cryopreservation did not improves viability and acrosomal membrane and mitochondrial integrity in boar sperm. Therefore, it is necessary to continue the study on the effects of ALA with carrier protein on boar spermatozoa during cryopreservation.

키워드

Alpha-linolenic acid BSA Methyl-β-Cyclodextrin Boar semen Cryopreservation

저자

  • Won-Hee Lee [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
  • Wook-Hwan Kim [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
  • Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
  • Boo-Keun Yang [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회

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