Earticle

초록

영어

Bisphenol-A (BPA) as an organic synthetic compound of exhibiting estrogen-mimicking and hormone-like properties, which is commonly used to induce cellular stress or female reproductive toxicity. In addition, BPA induces the increasing of mitochondrial derived reactive oxygen species (ROS) such as superoxide, and production of these ROS affects to the meiotic maturation and cumulus cells expansion on in vitro maturation (IVM) of porcine cumulus-oocyte complexes (COCs). However, anti-oxidative effect of melatonin for reduction of BPA-induced superoxide on porcine oocyte maturation has not been reported. Therefore, in present study, we confirmed that the reduction of BPA-derived superoxide by melatonin related to the reducing of mitochondria mediated apoptosis on meiotic maturation and cumulus cells expansion of porcine COCs. Then, to investigate the effects of superoxide specific scavenger, Mito-TEMPO, during porcine oocyte maturation progression, COCs cultured in maturation medium with Mito-TEMPO (0.1 μM) after pre-treatment of BPA (75 μM) for 22 h. Reduced meiotic maturation rate and cumulus cells expansion of COCs in the BPA (75 μM) treated group were recovered (p<0.01) by Mito-TEMPO treatment. Also, increasing of mitochondria derived apoptotic factors (AIF, Cleaved Caspase 3 and Cleaved PARP 1) protein levels by BPA treatment were reduced by Mito-TEMPO treatment in porcine COCs maturation. Positive effects of Mito-TEMPO for superoxide reduction on oocyte maturation and reducing mitochondrial apoptosis showed the same pattern in melatonin (0.1 μM) treated COCs. In case of supplemented with BPA and melatonin, superoxide production in COCs was not changed compared to control or melatonin treated groups. Based on these results, we concluded that melatonin as a regulator of superoxide such as Mito-TEMPO improves oocyte maturation through reduction of mitochondria derived apoptosis during IVM of porcine COCs.

키워드

저자

• Hyo-Jin Park [ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk 38453, Republic of Korea ]
• Jin-Woo Kim [ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk 38453, Republic of Korea ]
• Seul-Gi Yang1 [ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk 38453, Republic of Korea ]
• Min-Ji Kim [ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk 38453, Republic of Korea ]
• Ho-Guen Jegal [ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk 38453, Republic of Korea ]
• Joung Jun Park [ Animal Reproduction & Biotechnology Center, Myung-Poom Hanwoo Consulting, Gangwon 25232, Republic of Korea ]
• Deog-Bon Koo [ Department of Biotechnology, College of Engineering, Daegu University, Gyeongbuk 38453, Republic of Korea ]

참고문헌

발행기관

간행물

표지보기 관심저널 등록

• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636