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Poster Presentation : Oocyte Maturation / Embryonic Development

Effects of Serum Type in Maturation Medium on In Vitro Development of Bovine Embryos

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.90-90
  • 저자
    Ayman Mesalam, Shimin Zhang, Lianguang Xu, Myeong-Don Joo, Ji-Yoon Hwang, Kyeong-Lim Lee, Il-Keun Kong
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347476

원문정보

초록

영어
This study investigated the effect of Charcoal-Dextran Stripped fetal bovine serum (CDS FBS) and heat-inactivated FBS (HI FBS) in maturation medium on their ability to support in vitro oocyte maturation, cumulus cell-oocyte gap junctional communication, and development of bovine embryos. Oocytes were cultured in TCM-199 supplemented with either 8% BSA, 10% CDS FBS, or 10% HI FBS and 1 μg/mL estradiol-17β, 10 μg/mL FSH, 10 ng/mL EGF, 0.6 mM cysteine, 0.2 mM sodium pyruvate, and followed by in vitro fertilization and the zygotes were cultured in SOF-BE1 medium. The developmental ability and quality of bovine embryos were determined by assessing their cell number, lipid content, mitochondrial activity, gene expression, immunocytochemistry, and cryo-tolerance. The differences in embryo development between experimental groups were analyzed by one-way ANOVA. We have shown that CDS FBS had a higher significant (p<0.05) effect on the rate of blastocyst formation comparing to HI FBS and BSA (45.2±0.7% vs. 37.4±1.5% and 31.1±3.9%, respectively, six replicates were performed). Culture of oocytes with CDS FBS increased (p<0.05) the expression of gape junction proteins, CX37 and CX43, at both transcriptional and translation levels. We also found that CDS FBS significantly increased total cell number and decreased the apoptotic index in day-8 blastocyst comparing to BSA group. Furthermore, the beneficial effects of CDS FBS on embryos were associated with significantly reduced intracellular lipid content and increased mitochondrial activity in both oocytes and blastocyst. Taken together, these data suggest that supplementation of maturation medium with CDS FBS affect cumulus cell-oocyte gap junctional communication, and subsequently improved in vitro developmental competence of bovine oocytes and embryos.

키워드

CDS FBS HI FBS Gap junctional communication Embryo quality

저자

  • Ayman Mesalam [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Shimin Zhang [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Lianguang Xu [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Myeong-Don Joo [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Ji-Yoon Hwang [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Kyeong-Lim Lee [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Il-Keun Kong [ Department of Animal Science, Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회

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