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Poster Presentation : Oocyte Maturation / Embryonic Development

Effects of Myricetin on In Vitro Maturation and Embryo Development in Porcine Oocytes

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.88-88
  • 저자
    Ji-Eun Lee, Yong Hwangbo, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347474

원문정보

초록

영어
In many studies regarding in vitro maturation of mammalian oocytes, flavonoids have been used to improve maturation rate and developmental competence of in vitro-matured oocytes as an one of antioxidants. Myricetin is one of flavonoids that is present in the garlic, tea, and nuts. In human, treatment of myricetin enhanced motility and viability in spermatozoa in vitro. However, study regarding with relation ship betweem myricetin and mammalian oocytes has not been conducted. Therefore, the aim of this study was to investigate the effects of myricetin on the maturation and developmental competence of porcine oocytes. The cumulus-oocyte complexes (COCs) were incubated in IVM medium containing 0, 10, 50, and 100 μg/mL myricetin for 44 h. After 44 h of IVM, oocytes were inseminated with spermatozoa and clevage and blastocyst formation rates were evaluated 48 h and 168 h of IVF, respectively. Nuclear maturation stage was assessed by the aceto-orcein method. For measurement of oxidative stress status, intracellular reactive oxygen species (ROS) and glutathione (GSH) levels were measured using carboxy-DCFDA and cell tracker red, respectively. In results, oocytes in metaphase-II stage was no significant difference between control and myricetin treatment groups. However, treatment of myricetin significantly decreased oocyte population in germinal vesicle (GV) stage (p<0.05), whereas oocytes were arrested in germinal vesicle breakdown (GVBD) stage by myricetin. Intracellular GSH level in oocytes was higher in myricetin treatment groups than control group, but there were no significant difference. On the other hand, intracellular ROS level was not changed among the treatment groups. Interestingly, clevage rate was slightly reduced in 50 μg/mL myricetin treatment group compared with control group. Our findings suggested that treatment of myricetin during in vitro maturation of porcine oocytes could disrupt interaction between nucleic and cytoplasmic maturation. However, more studies regarding with mechanism of this phenomenon are needed.

키워드

Myricetin Oocyte maturation Embryo development Antioxidant Pigs

저자

  • Ji-Eun Lee [ College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
  • Yong Hwangbo [ College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
  • Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
  • Boo-Keun Yang [ College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
  • Choon-Keun Park [ College of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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