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Poster Presentation : Implantation / Uterine Biology

Effect of MMP-2 & -9 on the Improvement of Mouse Pregnancy Potential

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.79-79
  • 저자
    Shimin Zhang, Ayman Mesalam, Myeong-don Joo, Ji-Yoon Hwang, Seon-hwa Oh, Muhammad Idress, Hongyu Liu, Il-Keun Kong
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347467

원문정보

초록

영어
Matrix metalloproteinases (MMPs) comprise a family of zinc-dependent proteinases. Proteins of the MMPs family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development. Successful implantation is closely linked to the expression of MMPs which greatly influences ability of an embryo to degrade the basement membrane of the uterine epithelium, mainly composed of type IV collagen, and invade the uterine stroma. The purpose of this study was to determine the MMP-2 & -9, also known as type IV collagenase, concentrations in mouse uterine fluid during implantation window, investigate the roles of MMP-2 & -9 on mouse trophoblastic cells invasion in vitro and study the effect of MMP-2 & -9 cotransfer with mouse embryos on reproductive performances. Protein level was detected by Elisa kit and invasiveness was assessed by an invasion assay. Optimal concentrations of MMP-2 and -9 was co-transferred to 2.5 dpc recipients using the non-surgical embryo transfer. Our results showed that MMP-2 & -9 protein levels in mouse uterine fluid were significantly increased at 2.5 dpc. Moreover, in vitro treatment significantly promoted both spreading and invasion of mouse trophoblastic cells as compared with the non-treated. Embryo transfer results showed that MMP-9 cotransfer enhanced mouse implantation and pregnancy rate compared with the control and MMP-2 cotransfered groups, however, that was not significant. Taken together, our findings imply that MMP-9 cotransfer with embryos can regulate embryo invasion during preimplantation, which may have serious consequence on embryo implantation and can be applied in other mammals so far as human being.

키워드

MMP-2 & -9 Invasion assay Non-surgical embryo transfer Pregnancy performances

저자

  • Shimin Zhang [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Ayman Mesalam [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Myeong-don Joo [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Ji-Yoon Hwang [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Seon-hwa Oh [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Muhammad Idress [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Hongyu Liu [ Department of Animal Science, Division of Applied Life Science (BK21 Plus) ]
  • Il-Keun Kong [ Department of Animal Science, Division of Applied Life Science (BK21 Plus), Institute of Agriculture and Life Science, Gyeongsang National University, Jinju 52828, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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