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Poster Presentation : Implantation / Uterine Biology

Effect of Transforming Growth Factor-β on Plasminogen Activator mRNA Expression and Activity in Porcine Uterine Epithelial Cells.

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.77-77
  • 저자
    Su-Jin Kim, Yong Hwangbo, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347465

원문정보

초록

영어
As an one of multifunctional cytokine, transforming growth factor-β (TGF-β) induces various signaling pathway in cells via bind to their receptor. This action of TGF-β plays a crucial role in regulation of uterine environment for successful pregnancy in mammalians. Plasminogen activators system (PAs) is one of important physiological system for reproductive events such as oocyte maturation, acrosome reaction, fertilization, and uterine remodeling. It is consist of two-types of PAs (urokinase-type, uPA; tissue-type, tPA), uPA-specific receptor (uPAR), and type-1 PA inhibitor (PAI-1). In various type of cells, activity of these system was regulated by gonadotropin, cytokines, and hormones. Although both of TGF-β and PAs system play important role in uterine environment, relationship between TGF-β and PAs system was not fully understood. Therefore, this study was conducted to investigate regulation of PAs activity and mRNA expression by TGF-β in porcine uterine epithelial cells (pUECs). pUECs were isolated from uterine horn and cultured until 50% confluence. Then, cells were starved in serum-free medium for 24 hours and subsequently medium supplemented with TGF-β supplemented medium (0, 0.1, 10, 50, and 100 ng/mL) for 24 hours. The expression level of mRNA was analyzed using real-time PCR and PA activity assay was used for measurement of PA activity in supernatant. In results, PA activity was significantly decreased in TGF- β 10, 50, and 100 ng/mL treared group compared with control group (p<0.05). On the other hand, TGF-β did not affect to expression levels of uPA, tPA, uPAR and PAI-1 mRNA. In present study, although expression of uPA, tPA, uPAR, and PAI-1 mRNA were not influenced by TGF-β treatment, relative PA activity was reduced according to TGF-β concentration increased. Therefore, these results suggested that TGF-β regulate translation process for control the PA activity in porcine uetrine cells.

키워드

PA system TGF-β Uterine epithelial cells Pigs

저자

  • Su-Jin Kim [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
  • Yong Hwangbo [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
  • Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
  • Boo-Keun Yang [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회

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