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Poster Presentation : General Reproduction

Fetal Sex Determination in Dairy Cattle using DNA Isolated from Maternal Plasma

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.61-61
  • 저자
    Hyun-Joo Lim, Hyun Jong Kim, Ji Hwan Lee, Soo Bong Park, Seung Min Ha, Dong Hyun Lim, Tae IL Kim, Min Su Kim, Kyung Suk Lee, Changgwon Dang
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347451

원문정보

초록

영어
The development of methodological strategies that allow for the prediction of the fetal sex in cattle still remains a zoo-technical challenge. So far, different methods have been implemented to direct the breeding management in dairy cattle, giving producers an advantage in decision-making regarding the activity of planning and monetary gains. The objective of this study was to evaluate the use of next generation sequencing of fetal DNA in the maternal plasma of pregnant cattle in order to determine the sex of the fetus. The whole blood samples were collected from the five Holstein cows at 20-week of pregnancy. The serum plasma was harvested and the cell free DNA (cfDNA) was isolated in order to construct whole genome DNA library. The resulting DNA libraries were then paired-end sequenced (2×101 bp) with Illumina’s HiSeq 2500 instrument. The high quality, clean reads were mapped to the Bovine reference UMD 3.1 of X and Y chromosome separately, resulting an average depth and coverage of 3.54 and 86.2%. Our results revealed that four dairy cattle with male fetuses and 1 with a female fetus. Furthermore, it was concluded that fetal cells in maternal plasma can be used to predict the sex of the fetus in cattle. The detection of fetal DNA sequences is a reality and could reduce the risk of invasive techniques for certain fetal disorders in the near future.

저자

  • Hyun-Joo Lim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Hyun Jong Kim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Ji Hwan Lee [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Soo Bong Park [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Seung Min Ha [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Dong Hyun Lim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Tae IL Kim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Min Su Kim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Kyung Suk Lee [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Changgwon Dang [ Dairy Science Division, National Institute of Animal Science, RDA ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회

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