Hyun-Joo Lim, Hyun Jong Kim, Ji Hwan Lee, Soo Bong Park, Seung Min Ha, Dong Hyun Lim, Tae IL Kim, Min Su Kim, Kyung Suk Lee, Changgwon Dang
언어
영어(ENG)
URL
https://www.earticle.net/Article/A347451
원문정보
초록
영어
The development of methodological strategies that allow for the prediction of the fetal sex in cattle still remains a zoo-technical challenge. So far, different methods have been implemented to direct the breeding management in dairy cattle, giving producers an advantage in decision-making regarding the activity of planning and monetary gains. The objective of this study was to evaluate the use of next generation sequencing of fetal DNA in the maternal plasma of pregnant cattle in order to determine the sex of the fetus. The whole blood samples were collected from the five Holstein cows at 20-week of pregnancy. The serum plasma was harvested and the cell free DNA (cfDNA) was isolated in order to construct whole genome DNA library. The resulting DNA libraries were then paired-end sequenced (2×101 bp) with Illumina’s HiSeq 2500 instrument. The high quality, clean reads were mapped to the Bovine reference UMD 3.1 of X and Y chromosome separately, resulting an average depth and coverage of 3.54 and 86.2%. Our results revealed that four dairy cattle with male fetuses and 1 with a female fetus. Furthermore, it was concluded that fetal cells in maternal plasma can be used to predict the sex of the fetus in cattle. The detection of fetal DNA sequences is a reality and could reduce the risk of invasive techniques for certain fetal disorders in the near future.