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Poster Presentation : Gene Expression / Function
Effect of Cryoprotectant Solutions Treated with Hydroxypropyl Cellulose (HPC) Supplementation for Oocyte Vitrification in Bovine
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- 발생공학 국제심포지엄 및 학술대회 바로가기
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- 통권
- 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
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- 페이지
- pp.51-51
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A347445
원문정보
초록
- 영어
- Successful cryopreservation of bovine oocytes is a very important technology for research and commercial applications. However, the survival and development rate of vitrified- thawed oocytes is lower then non-vitrified oocytes. This study was confirmed the effect of cryoprotectant solutions treated with Hydroxypropyl cellulose (HPC) supplementation for oocyte vitrification in bovine. For vitrification, bovine MII oocytes were pretreated with EG10 (added 0, 10, 50 and 100 μg/mL HPC) for 5 min, exposed to EG30 (added 0, 10, 50 and 100 μg/mL HPC) for 30 sec, and then directly plunged into LN2. Thawing was taken by 4-step procedures [1 M sucrose and 10% FBS added D-PBS (SFD) → 0.5 M SFD → 0.25 M SFD → 0.125 M SFD ] for 1 min, respectively. After thawing, cumulus cells were partially removed by treatment with 0.05% hyaluronidaseand mechanical pipetting. Oocytes were washed with TL-HEPES, incubated in a droplet of previous cultured IVM medium for 1 h to recover. As shown results, in vitro survival rate of bovine vitrified-thawed MII oocyte was significantly higher in 50 (85.5%) and 100 μg/mL (80.2%) groups than 0 (71.2%) and 10 μg/mL (71.3%) groups. After in vitro fertilization, the cleavage rate was no significantly different between treatment groups. Therefore, these results were shown HPC treatment have a positive effect on the survival of vitrified-thawed bovine oocytes.
키워드
Vitrified-thawed oocyte IVF Bovine저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- 발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
- 간기
- 연간
- 수록기간
- 2004~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회
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