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Poster Presentation : Gene Expression / Function

Analysis of Bovine Interferon-tau Gene Subtypes Expression in the Trophoblast and Non-trophoblast Cells

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.45-45
  • 저자
    Min-Su Kim,, Hyun-Joo Lim, Ji Hwan Lee, Soo Bong Park, Hyun Jong Kim
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347439

원문정보

초록

영어
Interferon-tau (IFNT) is known as a major conceptus protein that signals the process of maternal recognition of pregnancy in ruminants. Also, multiple interferon (IFNT) genes exist in cattle, However, molecular mechanisms of these bIFNT genes whose expressions are limited have not been characterized. We and others have observed that expression levels of bovine subtype IFNT genes in the tissues of ruminants; thus, bIFNT1 and other new type I (bIFNTc1/c2/c3) gene co-exist during the early stages of conceptus development and non-trophoblast cells. Its genes transcription could be regulated through caudal-relatedhomeobox-2 (CDX2) and ETS2 and AP1(JUN) and/or cAMP-response element binding protein (CREB)-binding protein (CREBBP) expression, a transcription factor implicated in the control of cell differentiation in the trophectoderm. Bovine earderived fibroblast (EF) cells, were co-transfected with luciferase reporter constructs carrying upstream (positions −1000 to +51) regions of bIFNT1 and other new type I (bIFNT c1/c2/c3) gene and various transcription factor expression plasmids. Compared to each -1kb-bIFNT1/c1/c2/c3-Luc increased when this constructs were co-transfected with CDX2, ETS2, AP1 and/or CREBBP. Also, Its genes was had very effect on activity by CDX2, either alone or with the other transcription factors, markedly increased luciferase activity. However, the degree of transcriptional activation of the bIFNTc1 gene was not similar to that bIFNT1/c2/c3 gene by expression plasmid. Furthermore, RNA-seq analyses also revealed that the expression levels of bIFNT1/ c2/c3 gene mRNAs expression were highest on day 17, 20 and 22 trophoblast and Madin-Darby bovine kidney (MDBK), Bovine ear fibroblast (EF) and (Endo) non-trophoblast cells. But, bIFNTc1 mRNA had not same expression level, bIFNTc1 lowest levels than those of IFNT1/c2/c3 gene in both trophoblast and non-trophoblast cells. These results demonstrate that bovine subtype bIFNT genes display differential, in the trophoblast and non-trophoblast cells.

키워드

Interferon-tau Ruminants Transcription factors Trophoblast

저자

  • Min-Su Kim, [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Hyun-Joo Lim [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Ji Hwan Lee [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Soo Bong Park [ Dairy Science Division, National Institute of Animal Science, RDA ]
  • Hyun Jong Kim [ Dairy Science Division, National Institute of Animal Science, RDA ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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