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Poster Presentation : Cryopreservation

Effects of Leucosporidium Ice-binding Protein on the Cryopreservation of Boar Sperm

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.39-39
  • 저자
    Sang Hyoun Park, Keon Bong Oh, Sun-A Ock, Sung June Byun, Hwi-Cheul Lee, Hyeon Yang, Seongsoo Hwang, Gi-Sun Im, Jae-Seok Woo
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347435

원문정보

초록

영어
This study was performed to evaluate effects of Leucosporidium ice-binding protein (LeIBP) supplementation on boar (Duroc) sperm freezing. The collected semen was diluted (1.5×10⁸ /mL) in lactose egg yolk (LEY) and cooled at 5°C for 3 h. The cooled semen was then diluted (1×10⁸ /mL) in LeIBP containing LEY with 9% glycerol and maintained at 5°C for 30 min. The semen was divided into six experimental groups (control, 0.001, 0.005, 0.01, 0.05, and 0.1 mg/mL of LeIBP). The straws were stayed above the liquid nitrogen (LN2) vapors for 20 minutes and then plunged into LN2. After thawing, sperm parameters such as motility, viability, and acrosome integrity, intracellular reactive oxygen species (ROS), apoptosis, and undetected lipid peroxidation were determined. Computer assisted sperm analysis was used for sperm parameters and flowcytometry was performed to acrosome integrity (FITC-PSA/PI), ROS (DCFHDA/ PI), lipid peroxidation (BODIPY C11/PI), and apoptosis (Annexin V/PI), respectively. The differences were not in sperm motility, but in viability of 0.05 and 0.1 mg/mL groups compared to control (p<0.05). Higher acrosome integrity was observed in LeIBP groups compared to control (p<0.05), except for 0.001 group. Both ROS and lipid peroxidation level were lower in all LeIBP groups than that of control (p<0.05). Furthermore, lower apoptosis rate was observed in both 0.005 and 0.1 LeIBP groups compared to control (p<0.05). It can be postulated that a supplementation of LeIBP in boar sperm freezing extender is an effective method to increase the sperm qualities after cryopreservation.

키워드

Apoptosis Boar spermatozoa Cryopreservation Lipid peroxidation Leucosporidium ice-binding protein Reactive oxygen species

저자

  • Sang Hyoun Park [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Keon Bong Oh [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Sun-A Ock [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Sung June Byun [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Hwi-Cheul Lee [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Hyeon Yang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Seongsoo Hwang [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Gi-Sun Im [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]
  • Jae-Seok Woo [ Animal Biotechnology Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

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