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Poster Presentation : Cryopreservation
Establishment of Optimal Storage Temperature after Frozen-thawed of Black Goat Epididymal Spermatozoa.
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- 발행기관
- 한국동물생명공학회(구 한국동물번식학회) 바로가기
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- 간행물
- 발생공학 국제심포지엄 및 학술대회 바로가기
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- 통권
- 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
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- 페이지
- pp.37-37
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- 저자
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- 언어
- 영어(ENG)
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- URL
- https://www.earticle.net/Article/A347433
원문정보
초록
- 영어
- The purpose of this study was to observe the changes in motility after frozen-thawing of black goat epididymal sperm. The black goat testis was transported to the laboratory within 30 minutes from the slaughterhouse and the temperature was set at 36.5℃. Sperm were collected by pressing the sliced epididymis and used the Semen Washing Media. The egg yolk-triladyl frozen solution was used for the freezing of black goat sperm and the freezing concentration was set to 1×108 sperm/mL. The frozen sperm were thawed in water bath at 37.5℃ for 45 seconds and motility was measured after preservation for 0, 30, 60, 90 and 120 min at 4℃, 17℃ and 37℃, respectively. In results, sperm motility at 30 min after thawing was significantly higher in 17℃ than 37℃ (50.44% vs. 26.60%; p<0.05). Likewise, sperm motility at storage time 60, 90, and 120 min after thawing was significantly lower in frozen-thawed sperm stored at 37℃ groups than frozen-thawed sperm stored at 4℃ and 17℃ (p<0.05). Although there was no significant difference between frozen-thawed sperm stored at 4℃ and 17℃, motility in the frozen-thawed sperm stored at 17℃ was slightly higher than stored at 4℃. In conclusion, this results suggested that 4℃ and 17℃ temperature are more efficient for storage of frozen-thawed spermatozoa in black goat than 37℃. However, more researches are needed to establish the optimal conditions or proper supplementation for sperm preservation.
키워드
Black goat epididymal sperm Cryopreservation Motility Frozen-thawing저자
간행물 정보
발행기관
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- 발행기관명
- 한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
- 설립연도
- 1976
- 분야
- 농수해양>축산학
- 소개
- 동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.
간행물
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- 간행물명
- 발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
- 간기
- 연간
- 수록기간
- 2004~2018
- 십진분류
- KDC 527 DDC 636
이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회
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