Earticle

초록

영어

Porcine spermatogonial stem cells (SSCs) are a useful model for a successful gene modification to generate transgenic sperm, and have been considered as an crucial tool for biomedical research. However, there have been no report presenting transfection methods suitable for porcine SSCs. Accordingly, we report optimized condition using an electroporation for gene modification of SSCs isolated from porcine testes. As the results. the highest transfection efficiency was observed in porcine SSCs transfected with 1 μg transgenic vector with a single electric pulse from an electroporator at a voltage of 100 V and a capacitor setting of 250 μF with minimal cell death. Moreover, the transfection efficiency and cell viability were constant without consideration of plasmid size. Furthermore, we didn’t observe loss of spermatozoa differentiation potential in the transfected porcine SSCs. From these results, we customize electroporation condition to porcine SSCs for establishment of successful gene delivery system effectively introducing foreign DNA into the genome of porcine SSCs without any loss of the original porcine SSCs characteristics.

저자

• Min Seong Kim [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
• Min Hee Park [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
• Ji Eun Park [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea ]
• Jung Im Yun [ Institute of Animal Resources, Kangwon National University, Chuncheon 24341, Korea ]
• Jung Hoon Choi [ College of Veterinary Medicine and Kangwon National University, Chuncheon 24341, Korea ]
• Eunsong Lee [ College of Veterinary Medicine and Kangwon National University, Chuncheon 24341, Korea ]
• Seung Tae Lee [ Department of Animal Life Science, Kangwon National University, Chuncheon 24341, Korea, Department of Applied Animal Science, Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

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간행물

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• 간행물명

  발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]

• 간기

  연간

• 수록기간

  2004~2018

• 십진분류

  KDC 527 DDC 636