Earticle

현재 위치 Home

Oral Presentation

Effect of Exogenous Plasmiongen Activators and Their Inhibitor on In Vitro Fertilization in Pigs

첫 페이지 보기
  • 발행기관
    한국동물생명공학회(구 한국동물번식학회) 바로가기
  • 간행물
    발생공학 국제심포지엄 및 학술대회 바로가기
  • 통권
    2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회 (2018.06)바로가기
  • 페이지
    pp.13-13
  • 저자
    Yong Hwangbo, Ji-Eun Lee, Hee-Tae Cheong, Boo-Keun Yang, Choon-Keun Park
  • 언어
    영어(ENG)
  • URL
    https://www.earticle.net/Article/A347419

원문정보

초록

영어
The aim of present study was to confirm changes of results in in vitro fertilization (IVF) by two types of exogenous plasminogen activators (urokinase-type, uPA; tissue- type, tPA), and their inhibitor (PAI-1) and investigate the regulatory mechanism. the cumulus-oocyte complexes (COCs) were aspirated from 3~6 mm antral follicles and matured for 44 hours. Then, the cumulus cells were removed for fertilization and denuded oocytes were co-incubated with spermatozoa for 18~20 hours in IVF medium containing 100 pg/mL uPA, tPA or PAI-1. The number of sperm that bound to zona pellucida (ZP) and ZP solubility were measured using hoechst 33342 and 0.5% (w/v) pronase, respectively. Aceto-orcein stain was used to assess sperm penetration and sperm characteristics were analyzed by flow cytometry. In result, sperm penetration was increased by uPA and PAI-1 treatment than other groups (p<0.05). Interestingly, treatment of tPA enhanced ratio of monopsermic fertilization in penetrated zygotes population and reduced polyspermy than uPA groups (p<0.05). Similar to sperm penetration, number of penetrated sperm per zygote were higher PAI-1 treatment than tPA group (p<0.05). The number of ZP bounded sperm and ZP digestion time were lower in uPA and tPA-treated zygotes than other treatment groups (p<0.05) and ZP resistance was increased by treatment of PAI-1 (p<0.05). In results in sperm assay, acrosome reaction did not affected by all treatment in live and all sperm population. However, tPA treatment increased ratio of damaged sperm (p<0.05). These findings shown that exogenous PAs could affect to result of IVF via regulation ZP resistance, ZP binding, and sperm viability and two-types PAs differently regulated the fertility of boar spermatozoa.

키워드

In vitro fertilization Plasminogen activator Plasminogen activator inhibitor Pigs

저자

  • Yong Hwangbo [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
  • Ji-Eun Lee [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
  • Hee-Tae Cheong [ College of Veterinary Medicine, Kangwon National University, Chuncheon 24341, Korea ]
  • Boo-Keun Yang [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]
  • Choon-Keun Park [ College of Animal Life Sciences, Kangwon National University, Chuncheon 24341, Korea ]

참고문헌

자료제공 : 네이버학술정보

간행물 정보

발행기관

  • 발행기관명
    한국동물생명공학회(구 한국동물번식학회) [The Korean Society of Animal Reproduction and Biotechnology]
  • 설립연도
    1976
  • 분야
    농수해양>축산학
  • 소개
    동물번식생리학, 동물생명공학, 수의학, 인공수정 및 수정란이식을 이용한 동물개량에 관한 이론과 기술의 발전을 통해 학계, 연구계, 산업계 및 양축가 상호간의 협력을 도모함으로써 동물과학발전 및 사회일반의 이익에 기여 한다는 목적을 위해 노력해 나가겠습니다.

간행물

  • 간행물명
    발생공학 국제심포지엄 및 학술대회 [International Symposium on Developmental Biotechnology]
  • 간기
    연간
  • 수록기간
    2004~2018
  • 십진분류
    KDC 527 DDC 636

이 권호 내 다른 논문 / 발생공학 국제심포지엄 및 학술대회 2018년 한국동물번식학회, 한국수정란이식학회 공동학술대회

    피인용수 : 0건 (자료제공 : 네이버학술정보)

    함께 이용한 논문 이 논문을 다운로드한 분들이 이용한 다른 논문입니다.

      출처 : 네이버학술정보

        0개의 논문이 장바구니에 담겼습니다.

        페이지 저장
        소속기관 조회
        이용자님의 소속기관(단체)이 서비스에 가입되어 있는지 확인해 보십시오.
        기관회원에 소속되어 있는 이용자는 원문을 무료로 이용할 수 있습니다.